CAJ | 학술논문

测定了实验系统下固定化酶的本征动力学常数，从动力学角度分析研究了所开发研制的动态膜分离式固定化酶反应器（DMIR）的反应过程，给出了无外扩散限制条件下的DMIR反应器的数学模型，并验证了实验方法的可靠性。
측정료실험계통하고정화매적본정동역학상수，종동역학각도분석연구료소개발연제적동태막분리식고정화매반응기（DMIR）적반응과정，급출료무외확산한제조건하적DMIR반응기적수학모형，병험증료실험방법적가고성。
A Dynamic membrane-separation immobilized enzyme reactor(DMIR) was developed to apply in different operation processes successively. It is a type of continuous biological reactor which combines the immobilization of enzymes with membrane separation and which makes reaction and separation carry out simultaneously. To analyze and study the process of reaction-diffusion on the developing equipment，the kinetic parameters in various enzyme reaction systems and the intrinsic kinetic parameters of both free and immobilized glucoamylases were experimentally obtained. The experimental results showed that the rotating beds in the DMIR is superior to fixed bed as the presence of centrifugal field effectively enhances the conversion and production capacity, and in addition, it reduces or even eliminates the extra diffusion limitation. It was resulted in a inciease in the reaction rate of the immobilized enzyme. 　　The theoretical and experimental effective coefficients of different substance concentritions were calculated. A mathematics model of DMIR was provided without the condition of extra-diffusion limitation. Results have confirmed the reliability of the experimental method proposed in the thesis.