CAJ | 학술논문

以前的研究表明,高等植物叶绿体谷氨酰胺合成酶( GS2)受光调节,但叶片胞液GS(GS1)和非光合作用组织中的GS很少受光的影响。在本报道中, 笔者运用GS活性染色和Western blotting研究了光对非光合作用组织水稻根GS同工酶表达的影响。在阳光的直接照射下以及在室内不同光照强度下，可以很清楚地观察到GSra和GSrb的活性带及其蛋白质带。但是,当用尼龙网挡住阳光的直接照射时,GSrb的活性带和蛋白质带消失；当阳光被尼龙网遮挡住后,其光强度仍然比室内光照强度大得多,表明光照强度不是影响 GSrb表达的主要因素。当分析生长在暗处以及生长在光/暗转换下的水稻幼苗根GS同工酶变化时,仍然可以观察到GSrb的存在。在所有实验条件下,GSra都未发生明显变化。这些结果提示,光对GSrb表达的影响可能是由某些光谱相互作用所产生的未知因素造成的。
이전적연구표명,고등식물협록체곡안선알합성매( GS2)수광조절,단협편포액GS(GS1)화비광합작용조직중적GS흔소수광적영향。재본보도중, 필자운용GS활성염색화Western blotting연구료광대비광합작용조직수도근GS동공매표체적 영향。재양광적직접조사하이급재실내불동광조강도하，가이흔청초지관찰도GSra화GSrb적 활성대급기단백질대。단시,당용니룡망당주양광적직접조사시,GSrb적활성대화단백질대소 실；당양광피니룡망차당주후,기광강도잉연비실내광조강도대득다,표명광조강도불시영향 GSrb표체적주요인소。당분석생장재암처이급생장재광/암전환하적수도유묘근GS동공매변 화시,잉연가이관찰도GSrb적존재。재소유실험조건하,GSra도미발생명현변화。저사결과제 시,광대GSrb표체적영향가능시유모사광보상호작용소산생적미지인소조성적。
The previous studies showed that the express ion of chloroplast GS2 is mediated by light but cytosolic GS1 and GS in non-pho tosynthetic tissues of higher plants are little influenced.In the current report ,we have investigated that GS isozymes in rice roots respond to light using GS a ctivity staining and Western blotting.Both GSra and GSrb activity and their prot ein bands were detected at the illumination of sunlight and the incandescent lam p in the controlled environment chamber but GSrb activity and its protein bands were not observed under keeping out direct sunlight with the nylon net.The light intensity after the keeping out sunlight was higher than that of the incandesce nt lamp.This showed that light intensity was not a crucial factor for influencin g GSrb expression.The activity and the protein bands of GSrb in rice roots were observed still in the light/dark transition and the dark seedlings.GSra activity was not remarkably changed at the light treatment.The results suggest that the effect of light on the expression of GSrb isoform in rice roots may be achieved through some unknown factor(s) resulted from the interaction of some spectra.