遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2001年
1期
52-55
,共4页
陈军方%英加%王苏玲%刘朝晖%齐莉莉%陈佩度
陳軍方%英加%王囌玲%劉朝暉%齊莉莉%陳珮度
진군방%영가%왕소령%류조휘%제리리%진패도
普通小麦%簇毛麦%phlb突变体%端二体%C-分带%荧光原位杂交
普通小麥%簇毛麥%phlb突變體%耑二體%C-分帶%熒光原位雜交
보통소맥%족모맥%phlb돌변체%단이체%C-분대%형광원위잡교
用中国春phlb突变体(C.Sphlbphlb)与普通小麦柎-簇毛麦6V(6A)异代换系(Sub.6V)杂交,再用phlb突变体与F1回交,在高配对植株中筛选出一个6V染色体发生变化的植株,编号为LⅤ02。用染色体C-分带和荧光原位杂交技术,对LV02株系的后代进一步鉴定,在BC1F2中筛选鉴定出1株编号为LⅤ02-01的植株,该株含有40条普通小麦染色体,1条簇毛麦6V染色体和1条6V短臂端着丝粒染色体。在LⅤ02-01的分离后代中用同样技术鉴定出8株普通小麦-簇毛麦6VS端二体代换系。
用中國春phlb突變體(C.Sphlbphlb)與普通小麥柎-簇毛麥6V(6A)異代換繫(Sub.6V)雜交,再用phlb突變體與F1迴交,在高配對植株中篩選齣一箇6V染色體髮生變化的植株,編號為LⅤ02。用染色體C-分帶和熒光原位雜交技術,對LV02株繫的後代進一步鑒定,在BC1F2中篩選鑒定齣1株編號為LⅤ02-01的植株,該株含有40條普通小麥染色體,1條簇毛麥6V染色體和1條6V短臂耑著絲粒染色體。在LⅤ02-01的分離後代中用同樣技術鑒定齣8株普通小麥-簇毛麥6VS耑二體代換繫。
용중국춘phlb돌변체(C.Sphlbphlb)여보통소맥부-족모맥6V(6A)이대환계(Sub.6V)잡교,재용phlb돌변체여F1회교,재고배대식주중사선출일개6V염색체발생변화적식주,편호위LⅤ02。용염색체C-분대화형광원위잡교기술,대LV02주계적후대진일보감정,재BC1F2중사선감정출1주편호위LⅤ02-01적식주,해주함유40조보통소맥염색체,1조족모맥6V염색체화1조6V단비단착사립염색체。재LⅤ02-01적분리후대중용동양기술감정출8주보통소맥-족모맥6VS단이체대환계。
Chinese Spring phlb mutant (C S phlbphlb) was crossed to Triticumaestivum朒aynaldia villosa 6V (6A) alien substitution line and F1 back was crossed with C.S phlbphlb. One LⅤ02 with varied H. villosa 6V chromosome and one LⅤ02-01 with 40 T. aestivum chromosome, one H. villosa 6V and 6VS chromosome were screened in BC1F1 and BC1F2 respectively by C-banding and the fluorescence in situ hybridization (FISH). In segregated generation of LⅤ02-01, eight T.