CAJ | 학술논문

①目的探讨维生素 C对葡萄糖测定的干扰及其作用机制。②方法用葡萄糖氧化酶-过氧化物酶(GOD-PAP)法和己糖激酶(HK)法测定葡萄糖浓度。③结果用GOD-PAP法测定葡萄糖浓度相同、维生素C浓度不同的系列溶液时，所测葡萄糖浓度随着维生素C浓度的增高而降低；用HK法进行测定时，所测葡萄糖浓度基本恒定。用GOD-PAP法测定不含葡萄糖的过氧化氢溶液时，葡萄糖表观浓度随着过氧化氢稀释度的增大而降低；当过氧化氢浓度相同时，葡萄糖表观浓度随着维生素C浓度的增高而降低。④结论维生素C可能通过使反应的中间产物过氧化氢及最终产物苯醌亚胺还原而干扰GOD-PAP法对葡萄糖的测定结果。
①목적탐토유생소 C대포도당측정적간우급기작용궤제。②방법용포도당양화매-과양화물매(GOD-PAP)법화기당격매(HK)법측정포도당농도。③결과용GOD-PAP법측정포도당농도상동、유생소C농도불동적계렬용액시，소측포도당농도수착유생소C농도적증고이강저；용HK법진행측정시，소측포도당농도기본항정。용GOD-PAP법측정불함포도당적과양화경용액시，포도당표관농도수착과양화경희석도적증대이강저；당과양화경농도상동시，포도당표관농도수착유생소C농도적증고이강저。④결론유생소C가능통과사반응적중간산물과양화경급최종산물분곤아알환원이간우GOD-PAP법대포도당적측정결과。
Objective To study the interference and mechanism of vitamin C on the determination of glucose. Methods Glucose concentrations were determined with GOD-PAP method and HK method. Results When a series of solutions containing same concentration of glucose but different concentrations of vitamin C was determined with GOD-PAP method, the determined glucose concentration decreased with the increase of vitamin C concentration in the solution, but vitamin C did not affect the determination of glucose with HK method. When glucose-free hydrogen peroxide solution was determined with GOD-PAP method, the apparent glucose concentration decreased with dilution of hydrogen peroxide; with constant hydrogen peroxide concentration in the solutions, the apparent glucose concentration decreased with increase of vitamin C concentration in the solutions. Conclusion Vitamin C interferes with the determination of glucose possibly through the reduction of the intermittent product hydrogen peroxide and the final product quinoneimine.