CAJ | 학술논문

将类甜蛋白基因(TLP)及其紧密连锁的抗除草剂bar基因以农杆菌介导法导入脱毒微型种薯“津引薯8号”，经除草剂筛选后的转基因单株无性繁殖用于分子检测，标记基因bar基因的PCR反应和含TLP基因特异探针的Southern检测结果，转基因植株分别显示出0.54kb和3.0kb的特异性条带，证明TLP基因已成功地整合到转基因马铃薯基因组中。利用TLP基因特异性抗血清对转基因植株进行Western反应检测，结果表明，转基因植株中TLP基因表达量显著高于对照组，经晚疫病菌游离孢子接种离体抗性分析，转基因株系表现出对晚疫病的明显抑制作用和症状发生的延迟作用。
장류첨단백기인(TLP)급기긴밀련쇄적항제초제bar기인이농간균개도법도입탈독미형충서“진인서8호”，경제초제사선후적전기인단주무성번식용우분자검측，표기기인bar기인적PCR반응화함TLP기인특이탐침적Southern검측결과，전기인식주분별현시출0.54kb화3.0kb적특이성조대，증명TLP기인이성공지정합도전기인마령서기인조중。이용TLP기인특이성항혈청대전기인식주진행Western반응검측，결과표명，전기인식주중TLP기인표체량현저고우대조조，경만역병균유리포자접충리체항성분석，전기인주계표현출대만역병적명현억제작용화증상발생적연지작용。
Thaumatin-like protein (TLP)gene and bar g ene which is closelycombined with TLP gene were transferred into virus-free mi cropotato variety Jing Yinshu 8 by agrobacterium-mediated transformation.Transg enic plants selected by bialaphos were amplified for detection.The results of PCR of maker bar gene and Southern blot of specific probe with TLP gene show ed that transgenic plants displayed 0.54 kb and 3.0 kb bands,respectively,indica ting TLP gene has been successfully integrated into the chromosome of transgenic potatoes.The western reaction of transgenic plants using TLP antiserum showed the expression of TLP gene in transgenic plants was much higher than the control .Th rough the vitro assay of leaves inoculated by alive spores of phytophthora,leave s of transgenic potato plants exhibited significant inhibition on late-blight disease and delayed development of disease symptoms.