CAJ | 학술논문

在伪狂犬病病毒种特异性单克隆抗体的基础上建立了检测该病毒抗原的单抗夹心LAB-ELISA方法。结果表明，该方法不与其他常见病原体产生交叉反应，抗原的最低检出含量为8.9μg/mL。检测时最佳采样部位是猪脑及扁桃体。对人工感染兔、自然感染猪以及临床可疑病猪的检出率分别为75%，75%及72.7%，因此本方法是一种具有良好特异性及较好敏感性的诊断方法。
재위광견병병독충특이성단극륭항체적기출상건립료검측해병독항원적단항협심LAB-ELISA방법。결과표명，해방법불여기타상견병원체산생교차반응，항원적최저검출함량위8.9μg/mL。검측시최가채양부위시저뇌급편도체。대인공감염토、자연감염저이급림상가의병저적검출솔분별위75%，75%급72.7%，인차본방법시일충구유량호특이성급교호민감성적진단방법。
A sandwich LAB-ELISA wa s developed to detect Pseudorabies virus(PrV) on the basis of species-specific monoclonal antibodies in this study.The results showed that there was no cross- r eactions between the PrV and other common pathogens.The minimum concentration of the antigen that could be detected with this assay was 8.9 μg/mL.The most suit able organ to get samples was brain or tonsil.The positive rates to artificially infected rabbits,naturally infected swine and clinical dubious swine were 75%,7 5% and 72.7%.Therefore this assay was a suitable method with good specificity a nd sensitivity in the diagnosis of Pseudorabies.