CAJ | 학술논문

将带有二叶一心的小苗扦插于附加不同浓度NaCl的MS培养基中，研究不同番茄材料发根、植株生长以及POD酶酶谱及其活性的变化，并通过花粉管导入技术将野生番茄的总DNA导入到栽培种。研究结果表明，Lycopersicon pennellii LA716和Lycopersicon pimpinellifolium LA2184始终具有相对高的生根率，在150mmol/L NaCl胁迫10d时，相对发根率分别为86.8%和100%。参试的4份野生种，在附加有75mmol/L NaCl的培养基上的株高和叶片数均高于对照(无NaCl)，随着NaCl浓度的不断提高，株高和叶片数都下降，Lycopersicon pennellii LA716下降幅度最小。4份野生种的耐盐性明显优于2份栽培种，当NaCl浓度提高到300mmol/L时，4份野生种的鲜重可达对照的0.90、1.07、1.60和1.53倍，而2份栽培种则为对照的0.55和0.33倍。当NaCl浓度为75mmol/L时，Lycopersicon peruvianum LA111和Lycopersicon pennellii LA716材料POD酶酶带数多于对照2条和1条，Lycopersicon cheesmanii LA166和Lycopersicon pimpinellifolium LA2184则少于对照1条和3条。利用花粉管导入技术可以将野生番茄的DNA导入到栽培番茄。
장대유이협일심적소묘천삽우부가불동농도NaCl적MS배양기중，연구불동번가재료발근、식주생장이급POD매매보급기활성적변화，병통과화분관도입기술장야생번가적총DNA도입도재배충。연구결과표명，Lycopersicon pennellii LA716화Lycopersicon pimpinellifolium LA2184시종구유상대고적생근솔，재150mmol/L NaCl협박10d시，상대발근솔분별위86.8%화100%。삼시적4빈야생충，재부가유75mmol/L NaCl적배양기상적주고화협편수균고우대조(무NaCl)，수착NaCl농도적불단제고，주고화협편수도하강，Lycopersicon pennellii LA716하강폭도최소。4빈야생충적내염성명현우우2빈재배충，당NaCl농도제고도300mmol/L시，4빈야생충적선중가체대조적0.90、1.07、1.60화1.53배，이2빈재배충칙위대조적0.55화0.33배。당NaCl농도위75mmol/L시，Lycopersicon peruvianum LA111화Lycopersicon pennellii LA716재료POD매매대수다우대조2조화1조，Lycopersicon cheesmanii LA166화Lycopersicon pimpinellifolium LA2184칙소우대조1조화3조。이용화분관도입기술가이장야생번가적DNA도입도재배번가。
The possibility of using in vitro shoot culture to evaluate salt tolerance of different tomato species was determined. Shoot with 3 leaves grew in media supplemented with 0,75,150,300,450mmol/L NaCl, and rooting and growth were determined. The different degree of salt tolerance among species was clearly shown on the basis of the shoot rooting and growth. Studies on POD isoenzymes at 75mmol/L NaCl stress and without stress were carried out. Exogenous DNA extracted and purified from the leaves of wild tomato species was injected into the ovary of cultivated tomato about 24h after artificial self-pollination. Some characters of the donor were observed in offsprings. The results obtained in this paper showed Lycopersicon pennellii LA716 was the most salt-tolerant in vitro shoot culture and it could be a better system for testing and selecting for salt tolerance.