CAJ | 학술논문

目的:探讨不同反应靶细胞中白细胞介素6 (IL-6) 对信号转导子与转录激活子1 (STAT1) 的激活.方法:利用抗STAT1和抗酪氨酸磷酸化STAT1的抗体,进行免疫印迹分析.结果:IL-6可促进7TD1和TF1细胞生长,但在这两种细胞中对STAT1的酪氨酸磷酸化水平没有明显影响.M1、R2和U937细胞在IL-6诱导下生长停止并向巨噬细胞方向分化,而IL-6在这3种细胞中均可激活STAT1,且激活效应有剂量与时间依赖性.结论:STAT1的活化可能与抑制效应有关.我们的数据有助于解释为什么IL-6在不同效应靶细胞中有不同的生物学效应.
목적:탐토불동반응파세포중백세포개소6 (IL-6) 대신호전도자여전록격활자1 (STAT1) 적격활.방법:이용항STAT1화항락안산린산화STAT1적항체,진행면역인적분석.결과:IL-6가촉진7TD1화TF1세포생장,단재저량충세포중대STAT1적락안산린산화수평몰유명현영향.M1、R2화U937세포재IL-6유도하생장정지병향거서세포방향분화,이IL-6재저3충세포중균가격활STAT1,차격활효응유제량여시간의뢰성.결론:STAT1적활화가능여억제효응유관.아문적수거유조우해석위십요IL-6재불동효응파세포중유불동적생물학효응.
AIM: To investigate the activation status of signal transducer and activator of transcription 1 (STAT1) induced by interlerkin-6 (IL-6) in di fferent responsive cells. METHODS: Immunoblot analysis was p erformed with antibodies against STAT1 and tyrosine-phosphorylated STAT1. RESULTS: It ' s found that IL-6 had growth-promot ing effects on 7TD1 and TF1 cells but had little effect on the tyrosine-phosphor ylation of STAT1. On the other hand,IL-6 activated STAT1 in a dose- and time-de pendent manner in M1,R2 and U937 cells which underwent growth arrest and macrop hage differentiation upon IL-6 induction. CONCLUSION: These resu lts suggest that IL-6-induced STAT1 activation might be associated with growth-I nhibitory effects,and our data might help to explain why IL-6 exerts distinct biological effects in different target cells.