CAJ | 학술논문

目的探讨MAPKs激活在慢性醛固酮灌注诱发高血压肾损伤中的作用.方法6周SD大鼠随机分组为对照组,醛固酮/盐组及醛固酮拮抗剂eplerenone治疗组,采用Westem blotting技术检测各组大鼠肾皮质组织MAPKs亚型ERK1/2,JNK及p38MAPK活性.结果醛固酮/盐组大鼠血压显著升高,肾重/体重比值增加,尿蛋白排泄量增加,其肾皮质组织ERK1/2及JNK活性明显升高(P＜0.05).eplerenone治疗降低了醛固酮诱发的高血压,蛋白尿以及肾皮质MApKs活性.结论MAPKs激活与慢性醛固酮灌注致高血压肾损伤密切相关,Eplerenone其具有肾脏保护作用.
목적탐토MAPKs격활재만성철고동관주유발고혈압신손상중적작용.방법6주SD대서수궤분조위대조조,철고동/염조급철고동길항제eplerenone치료조,채용Westem blotting기술검측각조대서신피질조직MAPKs아형ERK1/2,JNK급p38MAPK활성.결과철고동/염조대서혈압현저승고,신중/체중비치증가,뇨단백배설량증가,기신피질조직ERK1/2급JNK활성명현승고(P＜0.05).eplerenone치료강저료철고동유발적고혈압,단백뇨이급신피질MApKs활성.결론MAPKs격활여만성철고동관주치고혈압신손상밀절상관,Eplerenone기구유신장보호작용.
Objective: To discuss the effect of mitogen-aetivated protein kinase (MAPKs) activation in aldosterone infused to induce the renal injury. Methods: 6 weeks Sprague-Dawley rats were divided into two groups.The control group: 1% NaC1 + vehicle (0.5% ethanol, n =8). The treatment group: 1% NaC1 + aldosterone (0.75% (g/rin, n =8), 1%NaCl + aldosterone + a selective mineralocorticoid receptor antagonist, eplerenone (0.125% in chow,n =8). The activities of MAPKs subfamilies, including ERK 1/2, JNK and p38-MAPK, in renal cortical tissues were measured by Western blot analysis. Results: Aldosterone-infused rats showed higher blood pressure and urinary excretion of protein increased. Renal cortical ERK 1/2 and JNK activities were significantly increased (P ＜0.05). Concurrent administration of eplerenone prevented the development of hypertension and elevation in urinary excretion of protein. Furthermore, eplerenone treatment normalized the activities of ERK 1/2 and JNK. Conclusions: These data support the concept that in aldosterone-infused hypertensive rats, MAPKs activation contributes to the progression of renal injury. A selective mineralocorticoid receptor antagonist, eplerenone showed renoprotective effects in this model.