CAJ | 학술논문

我们建立了由离体同步未分化颗粒细胞、动情前期垂体前叶和△-4雄烯二酮组成的"联合培养系统".该联合培养系统可模拟正常生理状态下各相关细胞间的协同作用,更有效地诱发经DES刺激后所获得的大量大鼠早期三级卵泡中的卵母细胞同步达到适当成熟,从而获得符合正常生理标准的成熟卵球,使卵巢内大量卵母细胞得到充分利用.我们的实验表明,经DES刺激,每侧幼龄大鼠卵巢中平均可取得处在早期三级卵泡中的189枚卵母细胞.在联合培养系统中培养,78%的卵母细胞排出第一极体达到成熟.这些成熟的卵母细胞中88%可正常受精,93%卵裂,经体外培养96h,有59%的受精卵可发育到桑椹或囊胚.2-细胞期胚胎移植后可发育为健康个体.因此,该联合培养系统为研究大鼠卵母细胞成熟、受精和早胚发育的分子机制提供了非常有效的实验模型.
아문건립료유리체동보미분화과립세포、동정전기수체전협화△-4웅희이동조성적"연합배양계통".해연합배양계통가모의정상생리상태하각상관세포간적협동작용,경유효지유발경DES자격후소획득적대량대서조기삼급란포중적란모세포동보체도괄당성숙,종이획득부합정상생리표준적성숙란구,사란소내대량란모세포득도충분이용.아문적실험표명,경DES자격,매측유령대서란소중평균가취득처재조기삼급란포중적189매란모세포.재연합배양계통중배양,78%적란모세포배출제일겁체체도성숙.저사성숙적란모세포중88%가정상수정,93%란렬,경체외배양96h,유59%적수정란가발육도상심혹낭배.2-세포기배태이식후가발육위건강개체.인차,해연합배양계통위연구대서란모세포성숙、수정화조배발육적분자궤제제공료비상유효적실험모형.
We have developed a "combination culture system", composed of primary differentiated granulosa cells, proestrus anterior pituitary lobe and Δ-4-androstenedione. This cultured system effectively induce the maturation of oocytes from early tertiary follicles of diethylstilbestrol (DES)-treated rats. After DES stimulation, an average of 189 oocytes were obtained from early tertiary follicles ofunilateral ovaries; 78% of these oocytes, when cultured in this culture system, extruded the first polar body, a criterion of oocyte maturation. Of the mature oocytes, the rate of normal fertilization and egg cleavage were 88% and 93%, respectively. After 96 h of culturing in vitro, 59% of the zygotes developed into morulae or blastocysts. Embryos implanted at the two-cell stage were able to develop into healthy individuals. Thus the combination culture system provides an experimental model in which the molecular mechanisms of egg maturation, fertilization, and embryonic development can be studied.