CAJ | 학술논문

目的研究血管紧张素转换酶(ACE)基因多态性及血清ACE水平与脑梗死之间的相互关系及可能机制.方法对84例脑梗死患者和74例健康对照者用聚合酶链反应(PCR)技术和琼脂糖凝胶电泳法分别进行ACE基因插入/缺失(I/D)多态性测定.分析比较脑梗死组与健康对照组之间ACE基因多态性的分布差异.结果脑梗死组DD基因型频率(50%)和D等位基因频率(64%)与健康对照组(分别为28%、46%)比较增高,差异有统计学意义(分别为P<0.01,P<0.05).ACE基因多态性与血清ACE水平有关,ACE水平依次为:DD型>ID型>Ⅱ型,三者相互之间差异有统计学意义(P<0.01).结论 ACE基因多态性与血清ACE水平和脑梗死有关,其DD型基因和D等位基因是脑梗死的危险因素.
목적 연구혈관긴장소전환매(ACE)기인다태성급혈청ACE수평여뇌경사지간적상호관계급가능궤제.방법 대84례뇌경사환자화74례건강대조자용취합매련반응(PCR)기술화경지당응효전영법분별진행ACE기인삽입/결실(I/D)다태성측정.분석비교뇌경사조여건강대조조지간ACE기인다태성적분포차이.결과 뇌경사조DD기인형빈솔(50%)화D등위기인빈솔(64%)여건강대조조(분별위28%、46%)비교증고,차이유통계학의의(분별위P<0.01,P<0.05).ACE기인다태성여혈청ACE수평유관,ACE수평의차위:DD형>ID형>Ⅱ형,삼자상호지간차이유통계학의의(P<0.01).결론 ACE기인다태성여혈청ACE수평화뇌경사유관,기DD형기인화D등위기인시뇌경사적위험인소.
Objective To investigate the association of angiotensin converting enzyme(ACE) gene polymorphism and its serum levels with cerebral infarction, and further to explore its possible mechanism. Methods Eighty-four patients with cerebral infarction(cerebral infarction group) and 74 healthy controls(healthy control group) underwent the determination of ACE gene insertion/deletion polymorphism by polymerase chain reaction(PCR) and agarose gel electrophoresis(AGE). The serum levels of ACE were measured simultaneously. The difference in distribution of ACE gene polymorphism was compared between cerebral infarction group and ealthy control group. Results The frequency of DD genotype in cerebral infarction group was significantly higher than that of healthy control group(50% vs 28% ,P<0.01); so was the frequency of D allele(64% vs 46% ,P<0.05). ACE gene polymorphism was associated with the serum level of ACE. A significant difference in serum ACE level was observed among three ACE genotypes. The highest serum ACE level or activity was in deletion homozygote(genotype DD), followed by heterzygote(genotype ID) and insertion homozygote(genotype Ⅱ)(P<0.01). Conclusion The polyrnorphism of ACE gene and serum level of ACE are associated with cerebral infarction. The DD gene type of ACE and D allele may be the risk factor for cerebral infarction.