CAJ | 학술논문

目的研究钩藤碱(Rhy)对颈动脉窦压力感受性反射(CSB)的作用并探讨其作用机制.方法利用隔离灌流大鼠颈动脉窦技术,确定各项功能参数,如阈压(TP)、饱和压(SP)、平衡压(EP)、工作范围(OR)、最大斜率(PS)和平均动脉压反射性下降幅度(RD)等.Rhy(10、50、100 μmol/L)溶于K-H液后灌流窦区,以斜坡方式升降窦内压来观察Rhy对CSB的影响.分别预先灌流一氧化氮合酶抑制剂L-NAME,钾通道阻断剂四乙铵(TEA)和L-型钙通道开放剂Bay K8644后再给予Rhy,观察它们对Rhy作用的影响.结果 Rhy浓度依赖性地抑制了CSB, 使其各功能参数改变, TP和SP增大,PS和RD均减小.Rhy的抑制作用不能被L-NAME(300 μmol/L)和 TEA(1 mmol/L)预处理所取消,而可被Bay K8644 (500 nmol/L)明显减弱.结论 Rhy抑制了CSB,此作用可能是通过减弱压力感受器的牵张敏感性离子通道的钙内流来实现,而与NO和钾通道无关.
목적 연구구등감(Rhy)대경동맥두압력감수성반사(CSB)적작용병탐토기작용궤제.방법 이용격리관류대서경동맥두기술,학정각항공능삼수,여역압(TP)、포화압(SP)、평형압(EP)、공작범위(OR)、최대사솔(PS)화평균동맥압반사성하강폭도(RD)등.Rhy(10、50、100 μmol/L)용우K-H액후관류두구,이사파방식승강두내압래관찰Rhy대CSB적영향.분별예선관류일양화담합매억제제L-NAME,갑통도조단제사을안(TEA)화L-형개통도개방제Bay K8644후재급여Rhy,관찰타문대Rhy작용적영향.결과 Rhy농도의뢰성지억제료CSB, 사기각공능삼수개변, TP화SP증대,PS화RD균감소.Rhy적억제작용불능피L-NAME(300 μmol/L)화 TEA(1 mmol/L)예처리소취소,이가피Bay K8644 (500 nmol/L)명현감약.결론 Rhy억제료CSB,차작용가능시통과감약압력감수기적견장민감성리자통도적개내류래실현,이여NO화갑통도무관.
Objective To study the effect of rhynchophylline (Rhy) on carotid sinus baroreflex (CSB) and the mechanism.Methods The technique of carotid sinus isolation and perfusion was used to determine the parameters of CSB, including threshold pressure (TP), saturation pressure (SP), equilibrium pressure (EP), operating range (OR), peak slope (PS) and reflex decrease (RD). The influence of Rhy on CSB was observed in a slope manner after Rhy (10, 50 and 100 μmol/L) dissolving in K-H solution and then filling into carotid sinus. Nitric oxide synthase inhibitor[N~G-nitro-arginine methyl ester(L-NAME)], potassium channel blocker [tetraethylammonium (TEA)] and agonist of L-type calcium channel (Bay K8644) were perfused into isolated carotid sinus before reperfusion of Rhy, and then the effects of them on Rhy were observed. Results Rhy inhibited CSB in a manner of dose-dependent with the increase of TP and SP and the decrease of PS and RD. The inhibitory effect of Rhy was not affected by the pretreatment of L-NAME (300 μmol/L) or TEA (1 mmol/L), but attenuated by Bay K8644 (500 nmol/L).Conclusion Rhy inhibits CSB, which may be related to that Rhy attenuates calcium influx of mechanosensitive ion channel of baroreceptor but not related to NO channel or potassium channel.