CAJ | 학술논문

凹叶厚朴是四川省人工栽种的一种用途广泛的常用中药.在发现凹叶厚朴乙醇提取物具有较强的α-葡萄糖苷酶抑制活性基础上,通过活性跟踪,对该提取物进行了分离,发现其中的生物碱显示了较强的抑制活性.通过溶剂萃取、树脂吸附和反复硅胶柱层析等分离方法从凹叶厚朴乙醇提取物中分离得到6个生物碱.用质谱和核磁共振等波谱方法分别鉴定为木兰箭毒碱,木兰花碱,鹅掌楸碱,蕃荔枝碱,罗默碱和Lysicamine,并应用小肠α-葡萄糖苷酶模型测定了它们对α-葡萄糖苷酶的抑制作用.其中,番荔枝碱和木兰箭毒碱的抑制活性较好,分别为60%和62%;其它4个生物碱成分活性几乎相当,鹅掌楸碱为46%,罗默碱为51%,Lysicamine为49%,木兰花碱为51%.图2参10
요협후박시사천성인공재충적일충용도엄범적상용중약.재발현요협후박을순제취물구유교강적α-포도당감매억제활성기출상,통과활성근종,대해제취물진행료분리,발현기중적생물감현시료교강적억제활성.통과용제췌취、수지흡부화반복규효주층석등분리방법종요협후박을순제취물중분리득도6개생물감.용질보화핵자공진등파보방법분별감정위목란전독감,목란화감,아장추감,번려지감,라묵감화Lysicamine,병응용소장α-포도당감매모형측정료타문대α-포도당감매적억제작용.기중,번려지감화목란전독감적억제활성교호,분별위60%화62%;기타4개생물감성분활성궤호상당,아장추감위46%,라묵감위51%,Lysicamine위49%,목란화감위51%.도2삼10
Magnolia officinalis Rehd. et Wils, an extensively cultivated traditional Chinese medicine in Sichuan, China, was widely used for curing various diseases. So far, there is no report about the inhibitory activity of this plant against a-glycosidase. The ethanol extract from M. officinalis showed a good inhibitory activity against α-glycosidase. The bio-guided separation showed that the alkaloids were the active components. By solvent extraction, resin absorption and repeated silica gel column chromatography, six known alkaloids as glycosidase inhibitor were isolated from M. officinalis and 6they were identified as liriodenine (1), anonaine (2), roemerine (3), lysicamine (4), magnoflorine (S) and magnocurarine (6) by spectroscopic methods. The inhibitory activity against a-glycosidase was determined in alvine screening model. Among them, anonaine and magnocurarine had better inhibiting activities (inhibition rates of 60% and 62%) than the other four alkaloids (from 46% to 51%). Fig 2, Ref 10