华中农业大学学报
華中農業大學學報
화중농업대학학보
JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY
2010年
2期
131-134
,共4页
新疆雪莲%愈伤组织%诱导%植物激素%外植体
新疆雪蓮%愈傷組織%誘導%植物激素%外植體
신강설련%유상조직%유도%식물격소%외식체
Saussurea involucrate%callus%induction%plant hormones%explants
以新疆雪莲叶片、叶柄和根为试验材料,研究了MS基本培养基附加不同质量浓度的植物激素对新疆雪莲愈伤组织诱导的影响.结果表明:单因素诱导中,NAA的诱导效果显著优于其它激素;以NAA为诱导激素,叶片与根,叶柄与根之间的诱导率存在极显著性差异,诱导效果:根＞叶柄＞叶片,最高诱导率分别为100%、98%、44%;NAA与6-BA激素组合对3种外植体愈伤组织的诱导中,叶片与叶柄诱导率之间存在极显著性差异,叶片、叶柄与根的诱导率之间存在显著性差异,诱导效果:叶片＞叶柄＞根,最高诱导率分别为98%、64%、23%.
以新疆雪蓮葉片、葉柄和根為試驗材料,研究瞭MS基本培養基附加不同質量濃度的植物激素對新疆雪蓮愈傷組織誘導的影響.結果錶明:單因素誘導中,NAA的誘導效果顯著優于其它激素;以NAA為誘導激素,葉片與根,葉柄與根之間的誘導率存在極顯著性差異,誘導效果:根＞葉柄＞葉片,最高誘導率分彆為100%、98%、44%;NAA與6-BA激素組閤對3種外植體愈傷組織的誘導中,葉片與葉柄誘導率之間存在極顯著性差異,葉片、葉柄與根的誘導率之間存在顯著性差異,誘導效果:葉片＞葉柄＞根,最高誘導率分彆為98%、64%、23%.
이신강설련협편、협병화근위시험재료,연구료MS기본배양기부가불동질량농도적식물격소대신강설련유상조직유도적영향.결과표명:단인소유도중,NAA적유도효과현저우우기타격소;이NAA위유도격소,협편여근,협병여근지간적유도솔존재겁현저성차이,유도효과:근＞협병＞협편,최고유도솔분별위100%、98%、44%;NAA여6-BA격소조합대3충외식체유상조직적유도중,협편여협병유도솔지간존재겁현저성차이,협편、협병여근적유도솔지간존재현저성차이,유도효과:협편＞협병＞근,최고유도솔분별위98%、64%、23%.
Using leaves,petioles and roots of Saussurea involucrate as explants,the effect of plant hormones on callus induction in MS medium was studied.The results showed that the medium containing NAA was better than other media.Comparing with whole explants,callus induction of leaves and roots,petioles and roots differed significantly.The inducing effect of roots,leaves and petioles was 100%,98% and 44%,respectively.Using the combination of NAA and 6-BA,callus induction of leaves,petioles and roots differs significantly as well.The induction effect of leaves,petioles and roots was 98%,64% and 23%,respectively.
