中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2010年
7期
918-920
,共3页
丹参酮%D-半乳糖%β-淀粉样肽蛋白%乙酰胆碱酯酶%海马
丹參酮%D-半乳糖%β-澱粉樣肽蛋白%乙酰膽堿酯酶%海馬
단삼동%D-반유당%β-정분양태단백%을선담감지매%해마
Tanshinone%D-galactose%β-amyloid peptide protein (Aβ)%Acetylcholinesterase (AChE)%Hippocampus
目的 探讨丹参酮对D-半乳糖-Aβ_(1-40)致复合痴呆模型大鼠海马内β-淀粉样蛋白(Aβ)和乙酰胆碱酯酶(AChE) 表达的影响.方法 实验动物随机分成AD模型组、丹参酮治疗组和溶媒对照组.采用免疫组织化学和酶组织化学方法 ,分别观察大鼠海马内Aβ和AChE表达的变化.结果 AD模型组大鼠海马内Aβ-ir细胞数显著增多,平均光密度增强;AChE阳性神经纤维受损,光密度下降(含阳性面积百分比和光密度);与溶媒对照组比较差异显著(P<0.01).丹参酮处理后,Aβ-ir细胞数明显减少,光密度也下降;AChE阳性神经纤维密度增强,与AD模型组比较差异有显著性意义(P<0.01).结论 丹参酮改善D-半乳糖-Aβ_(1-40)致复合痴呆大鼠学习记忆障碍的作用机制,可能与降低海马内Aβ的表达而保护脑内胆碱能神经有关.
目的 探討丹參酮對D-半乳糖-Aβ_(1-40)緻複閤癡呆模型大鼠海馬內β-澱粉樣蛋白(Aβ)和乙酰膽堿酯酶(AChE) 錶達的影響.方法 實驗動物隨機分成AD模型組、丹參酮治療組和溶媒對照組.採用免疫組織化學和酶組織化學方法 ,分彆觀察大鼠海馬內Aβ和AChE錶達的變化.結果 AD模型組大鼠海馬內Aβ-ir細胞數顯著增多,平均光密度增彊;AChE暘性神經纖維受損,光密度下降(含暘性麵積百分比和光密度);與溶媒對照組比較差異顯著(P<0.01).丹參酮處理後,Aβ-ir細胞數明顯減少,光密度也下降;AChE暘性神經纖維密度增彊,與AD模型組比較差異有顯著性意義(P<0.01).結論 丹參酮改善D-半乳糖-Aβ_(1-40)緻複閤癡呆大鼠學習記憶障礙的作用機製,可能與降低海馬內Aβ的錶達而保護腦內膽堿能神經有關.
목적 탐토단삼동대D-반유당-Aβ_(1-40)치복합치태모형대서해마내β-정분양단백(Aβ)화을선담감지매(AChE) 표체적영향.방법 실험동물수궤분성AD모형조、단삼동치료조화용매대조조.채용면역조직화학화매조직화학방법 ,분별관찰대서해마내Aβ화AChE표체적변화.결과 AD모형조대서해마내Aβ-ir세포수현저증다,평균광밀도증강;AChE양성신경섬유수손,광밀도하강(함양성면적백분비화광밀도);여용매대조조비교차이현저(P<0.01).단삼동처리후,Aβ-ir세포수명현감소,광밀도야하강;AChE양성신경섬유밀도증강,여AD모형조비교차이유현저성의의(P<0.01).결론 단삼동개선D-반유당-Aβ_(1-40)치복합치태대서학습기억장애적작용궤제,가능여강저해마내Aβ적표체이보호뇌내담감능신경유관.
Objective To investigate influence of tanshinone on levels of β-amyloid (Aβ) and acetylcholinesterase (AchE) in hippocampus of dementia model rats induced by D-galactose combination with Aβ1-40.Methods Rats were randomly divided into Alzheimer's disease (AD) model,tanshinone treatment,and solvent control groups. The expression of Aβ and AChE in rats hippocampus were respectively observed by immunohistochemical and enzyme-histochemical methods. Results Aβ-ir cells count and the average optical density of AD rats in the hippocampus were obviously increased,AChE positive nervous fibers damaged,optical density of AChE positive nervous fibers were significantly decreased in AD model group compared with solvent control group. After treated with tanshinone,Aβ-ir cells count and the optical density obviously decreased,AChE-positive nervous fibers were significantly increased compared with AD model group. Conclusions Mechanism of tanshinone on improving learning and memory impairment dementia model rats induced by D-galactose combination with Aβ1-40 might be related to decreasing expression of Aβ in hippocampus and protecting cholinergic nerves in brain.