CAJ | 학술논문

目的:建立以高效液相色谱法测定肉桂、木香CO_2超临界萃取物中桂皮醛、木香烃内酯和去氢木香内酯含量的方法.方法:色谱柱为Agilent HC-C_18(150 mm×4.6 mm,5 μm),流动相为乙腈-水(梯度洗脱),检测波长210 nm,流速1.0mL/min,柱温30℃.结果:桂皮醛进样量在148.5～1 732.5 ng与峰面积有良好的线性关系,平均回收率为99.65%,RSD为0.72%;木香烃内酯进样量在69.42～809.9 ng与峰面积有良好的线性关系,平均回收率为99.57%,RSD为1.28%;去氢木香内酯进样量在70.32～820.4ng与峰面积有良好的线性关系,平均回收率为98.90%,RSD为0.81%.结论:本方法简便、准确、重复性好,可用于肉桂、木香CO_2超临界萃取物的质量控制.
목적:건립이고효액상색보법측정육계、목향CO_2초림계췌취물중계피철、목향경내지화거경목향내지함량적방법.방법:색보주위Agilent HC-C_18(150 mm×4.6 mm,5 μm),류동상위을정-수(제도세탈),검측파장210 nm,류속1.0mL/min,주온30℃.결과:계피철진양량재148.5～1 732.5 ng여봉면적유량호적선성관계,평균회수솔위99.65%,RSD위0.72%;목향경내지진양량재69.42～809.9 ng여봉면적유량호적선성관계,평균회수솔위99.57%,RSD위1.28%;거경목향내지진양량재70.32～820.4ng여봉면적유량호적선성관계,평균회수솔위98.90%,RSD위0.81%.결론:본방법간편、준학、중복성호,가용우육계、목향CO_2초림계췌취물적질량공제.
AIM:To establish an HPLC method for determining cinnamaldehyde,costunolide and dehydrocostuslactone in the supercritical carbon dioxide extraction of Cinnamomum cassia and Aucklandia lapp.METHODS:The assay was performed on an Agilent HC-C_(18)(150 mm×4.6 mm,5 μm)column by UV detector at the wavelength of 210 nm with acetonitrile-water(gradient elutio)as the mobile phase at the flow rate of 1.0 mL/min,and the column temperature was 30℃.RESULTS:There were good relationships between peak area and sample size of cinnamaldehyde in the range of 148.5-1 732.5 ng,between peak area and sample size of costunolide in the range of 69.42-809.9 ng,and between peak area and sample size of dehydrocostuslactone in the range of 70.32 to 820.4 ng.Average recoveries of them were in turn 99.65%(RSD 0.72%)-99.57%(RSD 1.28%),and 98.90%(RSD 0.81%),respectively.CONCLUSION:The present method is convenient,sensitive and accurate with good reproducibility and can be used for the quality control of the supercritical CO_2 extract of Cinnamomum cassia and Aucklandia lapp.