CAJ | 학술논문

背景:干细胞因子作为对干细胞的增殖分化具有一定促进作用的因子,是否影响牙髓干细胞的生物学特性从而在牙齿再生中发挥重要作用尚不清楚.目的:观察干细胞因子对人牙髓干细胞增殖与骨向分化能力的影响.方法:采用酶消化法培养因正畸需要拔H{的健康人牙的牙髓组织,接种于培养板,加入含1,10 μmol/L干细胞因子的培养液,以正常培养液作为对照.MTT法检测细胞增殖,real-time PCR检测成骨相关基因骨唾液蛋白、骨钙素Mrna的表达,碱性磷酸酶试剂盒检测碱性磷酸酶活性.结果与结论:干细胞因子对牙髓干细胞的增殖具有促进作用,干细胞因子上调了骨唾液蛋白、骨钙素Mrna的表达,增强了牙髓干细胞的碱性磷酸酶活性,且具有随浓度增加促进作用增强的趋势.提示干细胞因子可以促进人牙髓干细胞的增殖及骨向分化能力.
배경:간세포인자작위대간세포적증식분화구유일정촉진작용적인자,시부영향아수간세포적생물학특성종이재아치재생중발휘중요작용상불청초.목적:관찰간세포인자대인아수간세포증식여골향분화능력적영향.방법:채용매소화법배양인정기수요발H{적건강인아적아수조직,접충우배양판,가입함1,10 μmol/L간세포인자적배양액,이정상배양액작위대조.MTT법검측세포증식,real-time PCR검측성골상관기인골타액단백、골개소Mrna적표체,감성린산매시제합검측감성린산매활성.결과여결론:간세포인자대아수간세포적증식구유촉진작용,간세포인자상조료골타액단백、골개소Mrna적표체,증강료아수간세포적감성린산매활성,차구유수농도증가촉진작용증강적추세.제시간세포인자가이촉진인아수간세포적증식급골향분화능력.
BACKGROUND:Stem cell factor(SCF)serves as a factor having a promotion effect on proliferation and differentiation of stemcells.Whether SCF affects biological features of dental pulp stem cells(DPSCs),and plays an important role in tooth regeneration remain unclear.OBJECTIVE:To observe SCF effects on the proliferation and osteogenic difierentiation of human DPSCs.METHODS:Pulp tissues were removed from healthy human teeth extracted for orthodontic purposes.The pulp tissues were digested by collagenase and dispase.The sample was cultivated in culture flasks with medium contained 1,1 0 μmol/L SCF;the normal culture medium served as control.The cell proliferation ability was detected by MTT.The osteogenic gene bone sialoprotein and osteocalcin mRNA expression were examined by real-time PCR.Alkaline phosphatase a ctivity was detectedr by alkaline phosphatase kit.RESULTS AND CONCLUSION:SCF could enhance the proliferation ability,upregulate bone sialoprotein and osteocalcin mRNA expression,and enhance the alkaline phosphatase activity,with the tendency of increased promotion with Increased concentration.Resuits indicated that SCF could enhance the proliferation and osteogenic difierentiation ability of human DPSCs.