中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
11期
1135-1137
,共3页
张晓玲%高菊梅%匡渤海%陈贺
張曉玲%高菊梅%劻渤海%陳賀
장효령%고국매%광발해%진하
卵巢子宫内膜异位症%间质细胞%增殖%凋亡%侵袭
卵巢子宮內膜異位癥%間質細胞%增殖%凋亡%侵襲
란소자궁내막이위증%간질세포%증식%조망%침습
Ovarian endometriosis%Stromal cell%Proliferation%Apoptosis%Invasion
目的 探讨卵巢子宫内膜异位症(OEMs)在位子宫内膜间质细胞的增殖、凋亡、侵袭能力.方法 体外培养并鉴定32例OEMs(OEMs组)和32例非OEMs患者(对照组)的在位子宫内膜间质细胞,应用MTT法、流式细胞仪、transwell法分别检测该细胞的增殖、凋亡和侵袭能力.结果 子宫内膜间质细胞体外培养成功,细胞鉴定纯度达90％以上；OEMs组细胞0时的吸光度值为0.127 ±0.014,对照组为0.129 ±0.008,两组比较差异无统计学意义(P＞0.05),OEMs组培养24、48 h增长的吸光度值与对照组比较差异均有统计学意义[24 h-0 h:0.148±0.020与0.048 ±0.008,t =26.066;48 h -24 h:0.397±0.029与0.119±0.022,t=42.544,P均＜0.01]；OEMs组在位子宫内膜间质细胞凋亡率为(26.430±3.789)％,对照组为(35.571 ±4.485)％,两组比较差异有统计学意义(t=-8.808,P＜0.01)；OEMs组在位子宫内膜间质细胞穿过小室后的吸光度值为0.950±0.014,对照组为0.653±0.028,两组比较差异有统计学意义(t=52.947,P＜0.01).结论 OEMs患者在位子宫内膜间质细胞的增殖、侵袭能力均高于对照组,凋亡能力低于对照组,这些细胞生物学特性的改变可能在OEMs的发生、发展中起作用.
目的 探討卵巢子宮內膜異位癥(OEMs)在位子宮內膜間質細胞的增殖、凋亡、侵襲能力.方法 體外培養併鑒定32例OEMs(OEMs組)和32例非OEMs患者(對照組)的在位子宮內膜間質細胞,應用MTT法、流式細胞儀、transwell法分彆檢測該細胞的增殖、凋亡和侵襲能力.結果 子宮內膜間質細胞體外培養成功,細胞鑒定純度達90％以上；OEMs組細胞0時的吸光度值為0.127 ±0.014,對照組為0.129 ±0.008,兩組比較差異無統計學意義(P＞0.05),OEMs組培養24、48 h增長的吸光度值與對照組比較差異均有統計學意義[24 h-0 h:0.148±0.020與0.048 ±0.008,t =26.066;48 h -24 h:0.397±0.029與0.119±0.022,t=42.544,P均＜0.01]；OEMs組在位子宮內膜間質細胞凋亡率為(26.430±3.789)％,對照組為(35.571 ±4.485)％,兩組比較差異有統計學意義(t=-8.808,P＜0.01)；OEMs組在位子宮內膜間質細胞穿過小室後的吸光度值為0.950±0.014,對照組為0.653±0.028,兩組比較差異有統計學意義(t=52.947,P＜0.01).結論 OEMs患者在位子宮內膜間質細胞的增殖、侵襲能力均高于對照組,凋亡能力低于對照組,這些細胞生物學特性的改變可能在OEMs的髮生、髮展中起作用.
목적 탐토란소자궁내막이위증(OEMs)재위자궁내막간질세포적증식、조망、침습능력.방법 체외배양병감정32례OEMs(OEMs조)화32례비OEMs환자(대조조)적재위자궁내막간질세포,응용MTT법、류식세포의、transwell법분별검측해세포적증식、조망화침습능력.결과 자궁내막간질세포체외배양성공,세포감정순도체90％이상；OEMs조세포0시적흡광도치위0.127 ±0.014,대조조위0.129 ±0.008,량조비교차이무통계학의의(P＞0.05),OEMs조배양24、48 h증장적흡광도치여대조조비교차이균유통계학의의[24 h-0 h:0.148±0.020여0.048 ±0.008,t =26.066;48 h -24 h:0.397±0.029여0.119±0.022,t=42.544,P균＜0.01]；OEMs조재위자궁내막간질세포조망솔위(26.430±3.789)％,대조조위(35.571 ±4.485)％,량조비교차이유통계학의의(t=-8.808,P＜0.01)；OEMs조재위자궁내막간질세포천과소실후적흡광도치위0.950±0.014,대조조위0.653±0.028,량조비교차이유통계학의의(t=52.947,P＜0.01).결론 OEMs환자재위자궁내막간질세포적증식、침습능력균고우대조조,조망능력저우대조조,저사세포생물학특성적개변가능재OEMs적발생、발전중기작용.
Objective To investigate eutopic endometrial stromal cells' ability of proliferation,apoptosis and invasion in ( ovarian endometriosis,OEMs).Methods Culture and identify eutopic endometrial stromal cells in 32 cases of patients with OEMs and 32 cases of patients with benign teratoma.Apply methyl thiazolyl tetrazolium methods,flow cytometric and transwell methods to detect these cells' capacity of proliferation,apoptosis and invasion.Results Eutopic endometrial stromal cells were cultured successfully and their purity was more than 90％.The zero hour's absorbance of OEMs group was similar with control group (0.127 ±0.013vs 0.129 ±0.008,P ＞ 0.05).The growth of 24 and 48 hour's absorbance of OEMs group was significantly higher than control group (24 h -0 h:0.148 ±0.020 vs 0.048 ±0.008,t =26.066;48 h -24 h:0.397 ±0.029vs 0.119 ±0.022,t =42.544,P ＜0.01 ).The apoptosis rate of eutopic endometrial stromal cells in OEMs group was (26.430 ± 3.789 )％ and ( 35.571 ± 4.485 ) ％ in control group,which reached statistical difference ( t =- 8.808,P ＜0.01 ).After the eutopic endometrial stromal cells got through the small room,the absorbance in OEMs group(0.950 ± 0.014) was significantly higher than control group (0.653 ± 0.028 ) ( t =52.947,P ＜0.01 ).Conclusion The proliferation and invasion capacity of eutopic endometrial stromal cell in OEMs group was more powerful than control group,however the apoptosis ability of this cell in OEMs group was weaker than control group.The change of biological characteristics of eutopic endometrial stromal cells of OEMs might be involved in the occurrance and development of OEMs.
