中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2012年
9期
650-655
,共6页
孙英新%张娟%于功昌%闫永建%陈雯雯%迟明峰%秦松
孫英新%張娟%于功昌%閆永建%陳雯雯%遲明峰%秦鬆
손영신%장연%우공창%염영건%진문문%지명봉%진송
百草桔%藻蓝蛋白%肺纤维化%转化生长因子-β1%NF-κB%肿瘤坏死因子-α
百草桔%藻藍蛋白%肺纖維化%轉化生長因子-β1%NF-κB%腫瘤壞死因子-α
백초길%조람단백%폐섬유화%전화생장인자-β1%NF-κB%종류배사인자-α
Paraquat%C-phycocyanin%Pulmonary fibrosis%Transforming growth factor%NF-Kappa B%Tumor necrosis factor
目的 探讨钝顶螺旋藻(spirulina platensis)藻蓝蛋白(C-phycoeyanin,C-PC)对百草枯(paraquat,PQ)染毒诱导大鼠肺纤维化的治疗作用.方法 选取健康Wistar大鼠90只,随机分为正常对照组、模型组(PQ组)和藻蓝蛋白治疗组(C-PC组),每组30只.PQ组、C-PC组以PQ(50 mg/kg)一次灌胃染毒造模.造模后,正常对照组、PQ组大鼠每天给予生理盐水(1 ml/100 g)灌胃,C-PC组每天给予C-PC (50 mg/kg)灌胃,并在第1、3、7、14、28天各组随机取6只大鼠右肺下叶肺组织匀浆测羟脯氨酸(HYP)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力；取左肺部分组织行HE染色、Masson染色进行病理观察,并用免疫组化方法观察肺组织中转化生长因子-β1(TGF-β1)、核因子-κB亚基p65(NF-κB p65)及肿瘤坏死因子-α(TNF-α)的表达.结果 各观察时点C-PC组大鼠肺组织中HYP和14、28 d MDA含量明显低于PQ组,差异有统计学意义(P＜0.05,P＜0.01).1、7、14、28 d时C-PC组大鼠肺组织中SOD活力明显高于PQ组,差异有统计学意义(P＜0.05,P＜0.01).PQ组和C-PC组肺组织中TGF-β1的表达及NF-κB p65和TNF-α表达明显高于正常对照组,C-PC组肺组织的TGF-β1表达及NF-κB p65和TNF-α表达明显低于PQ组,差异均有统计学意义(P＜0.05,P＜0.01).病理学观察显示C-PC能减轻PQ染毒大鼠肺泡炎和肺纤维化程度.结论 C-PC对PQ诱导的大鼠肺泡炎及肺纤维化有抑制作用.
目的 探討鈍頂螺鏇藻(spirulina platensis)藻藍蛋白(C-phycoeyanin,C-PC)對百草枯(paraquat,PQ)染毒誘導大鼠肺纖維化的治療作用.方法 選取健康Wistar大鼠90隻,隨機分為正常對照組、模型組(PQ組)和藻藍蛋白治療組(C-PC組),每組30隻.PQ組、C-PC組以PQ(50 mg/kg)一次灌胃染毒造模.造模後,正常對照組、PQ組大鼠每天給予生理鹽水(1 ml/100 g)灌胃,C-PC組每天給予C-PC (50 mg/kg)灌胃,併在第1、3、7、14、28天各組隨機取6隻大鼠右肺下葉肺組織勻漿測羥脯氨痠(HYP)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力；取左肺部分組織行HE染色、Masson染色進行病理觀察,併用免疫組化方法觀察肺組織中轉化生長因子-β1(TGF-β1)、覈因子-κB亞基p65(NF-κB p65)及腫瘤壞死因子-α(TNF-α)的錶達.結果 各觀察時點C-PC組大鼠肺組織中HYP和14、28 d MDA含量明顯低于PQ組,差異有統計學意義(P＜0.05,P＜0.01).1、7、14、28 d時C-PC組大鼠肺組織中SOD活力明顯高于PQ組,差異有統計學意義(P＜0.05,P＜0.01).PQ組和C-PC組肺組織中TGF-β1的錶達及NF-κB p65和TNF-α錶達明顯高于正常對照組,C-PC組肺組織的TGF-β1錶達及NF-κB p65和TNF-α錶達明顯低于PQ組,差異均有統計學意義(P＜0.05,P＜0.01).病理學觀察顯示C-PC能減輕PQ染毒大鼠肺泡炎和肺纖維化程度.結論 C-PC對PQ誘導的大鼠肺泡炎及肺纖維化有抑製作用.
목적 탐토둔정라선조(spirulina platensis)조람단백(C-phycoeyanin,C-PC)대백초고(paraquat,PQ)염독유도대서폐섬유화적치료작용.방법 선취건강Wistar대서90지,수궤분위정상대조조、모형조(PQ조)화조람단백치료조(C-PC조),매조30지.PQ조、C-PC조이PQ(50 mg/kg)일차관위염독조모.조모후,정상대조조、PQ조대서매천급여생리염수(1 ml/100 g)관위,C-PC조매천급여C-PC (50 mg/kg)관위,병재제1、3、7、14、28천각조수궤취6지대서우폐하협폐조직균장측간포안산(HYP)、병이철(MDA)함량、초양화물기화매(SOD)활력；취좌폐부분조직행HE염색、Masson염색진행병리관찰,병용면역조화방법관찰폐조직중전화생장인자-β1(TGF-β1)、핵인자-κB아기p65(NF-κB p65)급종류배사인자-α(TNF-α)적표체.결과 각관찰시점C-PC조대서폐조직중HYP화14、28 d MDA함량명현저우PQ조,차이유통계학의의(P＜0.05,P＜0.01).1、7、14、28 d시C-PC조대서폐조직중SOD활력명현고우PQ조,차이유통계학의의(P＜0.05,P＜0.01).PQ조화C-PC조폐조직중TGF-β1적표체급NF-κB p65화TNF-α표체명현고우정상대조조,C-PC조폐조직적TGF-β1표체급NF-κB p65화TNF-α표체명현저우PQ조,차이균유통계학의의(P＜0.05,P＜0.01).병이학관찰현시C-PC능감경PQ염독대서폐포염화폐섬유화정도.결론 C-PC대PQ유도적대서폐포염급폐섬유화유억제작용.
Objective To investigate the therapeutic effect of C-phycocyaniu (C-PC) from Spirulina platensis on paraquat (PQ)-induced pulmonary fibrosis in rats.Methods A total of 90 healthy Wistar rats were randomly and equally divided into normal control group,model group (PQ group),and C-PC treatment group (C-PC group).Each rat in the PQ group and C-PC group were orally administered with a single dose of PQ (50 mg/kg) to establish a rat model of PQ poisoning.Then,the rats in the normal control group and PQ group were orally given saline solution (1 ml/100 g) every day,and the rats in the C-PC group were orally given C-PC (50 mg/kg) every day.Six rats were randomly selected from each group on days 1,3,7,14,and 28.The inferior lobe of each rat's right lung was homogenized for the measurement of hydroxyproline (HYP) and maleic dialdehyde (MDA) levels and superoxide dismutase (SOD) activity.Parts of each rat's left lung were subject to HE staining and Masson staining for pathological observation,and the expression of transforming growth factor-β1 (TGF-β1),nuclear factor-kappa B p65 (NF-κB p65),and tumor necrosis factor-α (TNF-α) in lung tissue was measured by immunohistochemistry.Results The HYP levels on days 1,3,7,14,and 28 and MDA levels on days 14 and 28 were significantly lower in the C-PC group than in the PQ group (P＜0.05,P＜0.01).The SOD activity was significantly higher in the C-PC group than in the PQ group on days 1,7,14,and 28 (P＜0.05,P＜0.01).The protein content of TGF-β1 and the activities of NF-κB p65 and TNF-α in the PQ group and C-PC group were significantly higher than those in the normal control group,while the indices in the C-PC group were significantly lower than those in the PQ group (P＜0.05,P＜0.01).The pathological observation showed that C-PC could alleviate pulmonary alveolitis and fibrosis in rats with PQ poisoning.Conclusion C-PC can significantly inhibit PQ-induced pulmonary alveolitis and fibrosis in rats.
