CAJ | 학술논문

目的探讨神经颗粒素在系统性红斑狼疮(SLE)患者和正常人外周血单个核细胞(PBMCs)的表达状态.方法对比分析SLE PBMCs基因表达系列分析(SAGE)文库的高表达标签,并采用反转录聚合酶链反应(RT-PCR)法检测与正常人群有明显差异表达的神经颗粒素在SLE患者PBMCs的mRNA表达水平.结果对比分析SLE PBMCs和正常人各类淋巴细胞SAGE文库显示:神经颗粒素标签仅异位高表达于SLE患者,而几乎不表达于正常人各类淋巴细胞.RT-PCR检测证实:活动组SLE患者神经颗粒素mRNA表达水平较正常对照明显增加(P＜O.001),而缓解组SLE患者其表达水平仅稍有增加(P＞O.05).结论神经颗粒素作为一种前凋亡因子,高表达于SLE PBMCs,可能介导或参与SLE异常的免疫调节反应.
목적 탐토신경과립소재계통성홍반랑창(SLE)환자화정상인외주혈단개핵세포(PBMCs)적표체상태.방법 대비분석SLE PBMCs기인표체계렬분석(SAGE)문고적고표체표첨,병채용반전록취합매련반응(RT-PCR)법검측여정상인군유명현차이표체적신경과립소재SLE환자PBMCs적mRNA표체수평.결과 대비분석SLE PBMCs화정상인각류림파세포SAGE문고현시:신경과립소표첨부이위고표체우SLE환자,이궤호불표체우정상인각류림파세포.RT-PCR검측증실:활동조SLE환자신경과립소mRNA표체수평교정상대조명현증가(P＜O.001),이완해조SLE환자기표체수평부초유증가(P＞O.05).결론 신경과립소작위일충전조망인자,고표체우SLE PBMCs,가능개도혹삼여SLE이상적면역조절반응.
Objective To investigate the mRNA expression level of neurogranin on peripheral blood mononuclear cells(PBMCs)from patients with systemic lupus erythematosus(SLE).Methods Top 20 tags of SLE PBMCs SAGE library were searched from normal lymphocytes SAGE library including navie-T,Th1,Th2, CD8+T, NK and B cells,and their abundance was compared.The mRNA expression level of neuro-granin,a differential over-expressed tag,was detected in 35 cases of SLE and 15 normal controls by reversetranscription-polymerase chain reaction (RT-PCR).Results Neurogranin tag could only be detected in SLE PBMCs SAGE library,but was hardly found in normal lymphocyte SAGE library.However,either SLE pa-tients or normal controls showed a detectable mRNA level of neurogranin on PBMCs by RT-PCR.The mRNA level of neurogranin in active SLE patients was significantly increased than those in controls(P＜0.001).but only slightly increased in inactive SLE patients (P＞0.05).Conclusion Neurogranin,as a novel proapototic factor,is overexpressed on PBMCs of SLE patients.It may be involved in the regulation of abnormal immune responses in lupus.