CAJ | 학술논문

目的探讨水飞蓟素对肾间质纤维化大鼠血管紧张素Ⅱ(AngⅡ)表达的影响.方法 56只雄性SD大鼠完全随机分为假手术组8只,梗阻组24只,治疗组24只.梗阻组和治疗组大鼠利用单侧输尿管结扎法诱导肾小管间质纤维化模型,治疗组于治疗前30 min开始给予水飞蓟素30 mg/(kg·d)灌胃,梗阻组和假手术组同期给予生理盐水灌胃.梗阻组和治疗组分别于实验第7、14、21天各处死大鼠8只,假手术组于第21天处死全部大鼠,术侧肾组织观察病理变化并应用免疫组织化学方法检测肾组织AngⅡ的表达.结果假手术组、梗阻组与治疗组第21天时肾小管间质评分为(0.14±0.09)、(2.49±0.26)、(1.89±0.24)分;AngⅡ的表达分别为(0.24±0.07)%、(12.26±0.18)%、(8.48±0.44)%;梗阻组与治疗组各指标明显高于假手术组,治疗组低于梗阻组.结论水飞蓟素可能是通过下调AngⅡ表达而抑制纤维化的发生,从而起到对肾脏的保护作用.
목적 탐토수비계소대신간질섬유화대서혈관긴장소Ⅱ(AngⅡ)표체적영향.방법 56지웅성SD대서완전수궤분위가수술조8지,경조조24지,치료조24지.경조조화치료조대서이용단측수뇨관결찰법유도신소관간질섬유화모형,치료조우치료전30 min개시급여수비계소30 mg/(kg·d)관위,경조조화가수술조동기급여생리염수관위.경조조화치료조분별우실험제7、14、21천각처사대서8지,가수술조우제21천처사전부대서,술측신조직관찰병리변화병응용면역조직화학방법검측신조직AngⅡ적표체.결과 가수술조、경조조여치료조제21천시신소관간질평분위(0.14±0.09)、(2.49±0.26)、(1.89±0.24)분;AngⅡ적표체분별위(0.24±0.07)%、(12.26±0.18)%、(8.48±0.44)%;경조조여치료조각지표명현고우가수술조,치료조저우경조조.결론 수비계소가능시통과하조AngⅡ표체이억제섬유화적발생,종이기도대신장적보호작용.
Objective To investigate the effects of silymarin on the expression of Angiotensin Ⅱ in the kidneys of rats ith unilateral ureteral obstruction(UUO) -induced renal fibrosis. Methods Fifty-six rats were randomly assigned to shame peration group,operation group and silymarin[30 mg/(kg·d)] treatment group after unilateral ureteral obstruction. Tubulointerstitial fibrosis model was established via unilateral ureteral obstruction(UUO).Renal tissues were examined by light microscopy 7,14,21 days after operation. Immunhistochemistry was applied to measure the expression of Angiotensin Ⅱ in the renal interstitium. Results Silymarin may significantly ameliorate pathological change of nephridial tissue after unilateral ureteral obstruction. Silymarin may down-regulate the expression of Angiotensin Ⅱ in renal tissue. Conclusion Silymarin may inhibit renal fibrosis by down-regulating Angiotensin Ⅱ expression,which may play a key role in protection against renal fibrosis.