CAJ | 학술논문

目的研究高压氧治疗对脑创伤大鼠神经行为和突触素表达的影响.方法 24只Sprague Dawley (SD)大鼠按数字表法随机分为3组,即假手术组、脑创伤组(采用50 g重锤30 cm高度自由落体撞击制备大鼠运动皮质区脑损伤模型)和高压氧治疗组[脑创伤+高压氧治疗(每天1次,连续治疗7d)],每组8只.术后13d对各组大鼠进行神经功能缺损(neurological severity score,NSS)评分,观察动物运动和平衡功能缺损及改进情况；免疫组织化学方法检测各组脑组织突触素的表达情况.结果大鼠脑创伤后出现不同程度抽搐、瘫痪和平衡功能缺失.NSS评分结果显示:与假手术组(0.31±0.11)比较,脑创伤组NSS评分(4.11±0.50)明显升高,差异有统计学意义(P＜0.05)；高压氧治疗组(3.07±0.45)较脑创伤组NSS评分明显减少,差异有统计学意义(P＜0.05).免疫组化染色结果显示:脑创伤组脑组织突触素阳性表达数量(71±10)较假手术组(50±11)明显增加,差异有统计学意义(P＜0.05)；高压氧治疗组突触素阳性表达数量(89±13)较脑创伤组明显增加,差异有统计学意义(P＜0.05).结论高压氧治疗能促进脑创伤大鼠神经功能恢复,其作用机制可能与突触素表达增加有关.
목적 연구고압양치료대뇌창상대서신경행위화돌촉소표체적영향.방법 24지Sprague Dawley (SD)대서안수자표법수궤분위3조,즉가수술조、뇌창상조(채용50 g중추30 cm고도자유락체당격제비대서운동피질구뇌손상모형)화고압양치료조[뇌창상+고압양치료(매천1차,련속치료7d)],매조8지.술후13d대각조대서진행신경공능결손(neurological severity score,NSS)평분,관찰동물운동화평형공능결손급개진정황；면역조직화학방법검측각조뇌조직돌촉소적표체정황.결과 대서뇌창상후출현불동정도추휵、탄탄화평형공능결실.NSS평분결과현시:여가수술조(0.31±0.11)비교,뇌창상조NSS평분(4.11±0.50)명현승고,차이유통계학의의(P＜0.05)；고압양치료조(3.07±0.45)교뇌창상조NSS평분명현감소,차이유통계학의의(P＜0.05).면역조화염색결과현시:뇌창상조뇌조직돌촉소양성표체수량(71±10)교가수술조(50±11)명현증가,차이유통계학의의(P＜0.05)；고압양치료조돌촉소양성표체수량(89±13)교뇌창상조명현증가,차이유통계학의의(P＜0.05).결론 고압양치료능촉진뇌창상대서신경공능회복,기작용궤제가능여돌촉소표체증가유관.
Objective To study the effects of hyperbaric oxygen (HBO) on neurological behavior and synaptophysin expression in rats with traumatic brain injury (TBI).Methods Twenty-four Sprague Dawley (SD) rats were randomly divided into 3 groups:the sham surgery group (the control group),the TBI group (the animal model was developed with the 40 g weight-free-drop from a height of 30 cm),and the HBO treatment group (TBI + HBO treatment,once a day for 7 successive days),each consisting of 8 animals.Neurological severity score (NSS) evaluation was made to determine the disorder and improvement of neurological functions of all the animal groups,13 days after surgery.Immunocytochemistry was performed to detect the expression of synaptophysin in the brain tissues of all the animal groups.Statistical analysis was made with the single-factor t test.Results The affected animals displayed convulsion,paralysis and disequilibrium to various extents,following TBI.Results showed that NSS scores of the TBI group elevated obviously (4.11 ±0.50),with statistical significance,when compared with those of the sham surgery group (0.31 ± 0.11) ( P＜0.05 ).Results of immunocytochemistry indicated that there was a positive expression of synaptophysin in the TBI group,with a significant increase in the amount of the expression (71 ± 10),when compared with that of the sham surgery group (50 ± 11),with statistical significance ( P＜0.05 ).For the HBO treatment group,NSS scores (3.07 ±0.45) decreased considerably,when compared with those of the TBI group,with statistical significance ( P ＜ 0.05 ).The amount of synaptophysin expression in the HBO group( 89 + 13 ) was significantly higher than that of the TBI group,with statistical significance ( P ＜ 0.05 ).Conclusions HBO treatment could promote recovery of neurological functions of the rats with TBI and the increased expression of synaptophysin might be involved in its mechanism.