CAJ | 학술논문

目的分析237例HIV/AIDS患者中原发性耐药的发生率和病毒亚型分布情况.方法从河南、云南、上海等20个地区采集237例未经抗病毒治疗的HIV/AIDS患者血浆HIV-1进行基因测序,通过病毒亚型分析软件REGA HIV-1 Subtyping Tool确定测序样本的HIV-1亚型,并与斯坦福大学HIV耐药数据库比对确定测序样本HIV-1耐药相关突变位点.采用b-DNA方法检测患者血浆病毒载量,采用流式细胞仪检测CD4+ T淋巴细胞计数.结果 237例未接受抗病毒治疗的HIV/AIDS患者感染的HIV-1毒株分布为A1、B、C、CRF01-AE、CRF02-AG、CRY7-BC、CRF08-BC、CRF12-BF、G等9个不同亚型,可能的感染时间分布在1990-2006年.237例患者中仅有3例分别对核苷类逆转录酶抑制剂高度耐药、蛋白酶类抑制剂低度耐药和非核苷类逆转录酶抑制剂高度耐药,原发性耐药的发生率为1.3%(3/237).结论我国部分地区未经抗病毒治疗的HIV/AIDS患者中HIV-1原发性基因型耐药的发生比例处于较低水平,病毒亚型分布在9个亚型.
목적 분석237례HIV/AIDS환자중원발성내약적발생솔화병독아형분포정황.방법 종하남、운남、상해등20개지구채집237례미경항병독치료적HIV/AIDS환자혈장HIV-1진행기인측서,통과병독아형분석연건REGA HIV-1 Subtyping Tool학정측서양본적HIV-1아형,병여사탄복대학HIV내약수거고비대학정측서양본HIV-1내약상관돌변위점.채용b-DNA방법검측환자혈장병독재량,채용류식세포의검측CD4+ T림파세포계수.결과 237례미접수항병독치료적HIV/AIDS환자감염적HIV-1독주분포위A1、B、C、CRF01-AE、CRF02-AG、CRY7-BC、CRF08-BC、CRF12-BF、G등9개불동아형,가능적감염시간분포재1990-2006년.237례환자중부유3례분별대핵감류역전록매억제제고도내약、단백매류억제제저도내약화비핵감류역전록매억제제고도내약,원발성내약적발생솔위1.3%(3/237).결론아국부분지구미경항병독치료적HIV/AIDS환자중HIV-1원발성기인형내약적발생비례처우교저수평,병독아형분포재9개아형.
Objective To investigate the prevalence of primary HIV-1 genotype drug-resistance and viral subtype from 237 treatment-naive patients in China.nethods CD4+ T cell counts,plasma HIV-1 viral load and HIV-1 gene sequencing in total 237 treatment-naive patients enrolled from 20 provinces/regions were detected for the evaluation of primary HIV genotypic drug-resistance.Results The survey of 237 treatment-naive patients from muhicenter areas including Henan Province,Yunan Province,and Shanghai showed that 9 subtypes of HIV-1 strains were finally identified.Most of patients were infected before 2003.Only 3 cases had genotypic mutations associated resistance to antiretroviral drugs,with high resistance to nucleoside reverse transcriptase inhibitors(NRTIs),moderate resistance to protease inhibitors(PIs)and high resistance to non-nucleoside reverse transcriptase inhibitors(NNRTIs).respectively.The prevalence of primary genotypic drug resistance was 1.3%(3/237)in this study.Conclusions The rate of HIV-1 primary genotypic drug-resistance is still relatively low in treatment-naive HIV/AIDS patients while 9 subtypes of HIV-1 strain was diseovered.