CAJ | 학술논문

将鸭源鸡新城疫（ND）病毒D10株和鸡减蛋综合症（EDS-76）病毒GC2株同时接种于同一鸭胚内复制，增殖96 h收取尿囊液，用甲醛灭活后加白油佐剂制成ND-EDS-76异源二联油乳剂灭活疫苗。该疫苗生产工艺简单，成本低,经试用表明可有效地预防ND和EDS-76的发生。
장압원계신성역（ND）병독D10주화계감단종합증（EDS-76）병독GC2주동시접충우동일압배내복제，증식96 h수취뇨낭액，용갑철멸활후가백유좌제제성ND-EDS-76이원이련유유제멸활역묘。해역묘생산공예간단，성본저,경시용표명가유효지예방ND화EDS-76적발생。
Duck origin ND.D10 strain and EDS-76GC2 strain were inoculated into the same duck embryos and harvested after 96 hours of culture.The propagated virus were inactivated by formalin and mingled with oil emulsion adjuvant according to rational rate,and a combined inactivated oil-adjuvant duck origin NDV and EDS-76 vaccine was developed,which simplified the production process and reduced production cost.The tests of vaccine safety,antibody response,protective rate and duration of immunity were also preformed.The results show that this kind of vaccine could prevent ND virus and EDS-76 virus infection successfully.