CAJ | 학술논문

用免疫荧光、激光共聚焦显微镜图像分析及膜片钳等技术研究了鼻咽癌上皮CNE-2Z细胞容积激活性氯通道候选基因C1C-3的表达及其在细胞周期中与容积激活性氯电流及细胞容积调节性回缩(regulatoryvolumedecrease,RVD)的关系.结果显示,CNE-2Z细胞表达ClC-3.ClC-3蛋白主要位于细胞内而不是在细胞膜上,其表达水平及其在细胞中的分布呈细胞周期依赖性.G1期细胞的ClC-3表达水平较低而S期则较高,M期细胞的表达水平中等.在细胞周期中,ClC-3表达水平与细胞RVD能力及容积激活性氯电流水平呈反比.上述观察结果提示,ClC-3可能参与细胞周期的调节,但CNE-2Z细胞中的ClC-3可能不是与RVD有关的氯通道.
용면역형광、격광공취초현미경도상분석급막편겸등기술연구료비인암상피CNE-2Z세포용적격활성록통도후선기인C1C-3적표체급기재세포주기중여용적격활성록전류급세포용적조절성회축(regulatoryvolumedecrease,RVD)적관계.결과현시,CNE-2Z세포표체ClC-3.ClC-3단백주요위우세포내이불시재세포막상,기표체수평급기재세포중적분포정세포주기의뢰성.G1기세포적ClC-3표체수평교저이S기칙교고,M기세포적표체수평중등.재세포주기중,ClC-3표체수평여세포RVD능력급용적격활성록전류수평정반비.상술관찰결과제시,ClC-3가능삼여세포주기적조절,단CNE-2Z세포중적ClC-3가능불시여RVD유관적록통도.
The immunofluorescence approach, the confocal microscopy and the patch-clamp technique were used to investigate the expression of ClC-3 (one of the candidates of volume-activated chloride channels) and its relationships with the volume-activated chloride current and the capacity of regulatory volume decrease (RVD) in the cell cycle of nasopharyngeal carcinoma cells (CNE-2Z cells). The results indicated that CNE-2Z cells expressed ClC-3. ClC-3 was located predominantly inside the cells but not in the membrane. Both the expression level and the distribution of ClC-3 were cell cycle dependent. ClC-3 expression was low in G1 but high in S phase. The cells in G2/M phase possessed an intermediate level of the expression. ClC-3 expression level was negatively correlated to the RVD capacity and amplitude of the volume-activated chloride current in the cell cycle. The results suggest that C1C-3 may be an important factor in the regulation of cell cycle progression, but that it is probably not the chloride channel associated with RVD in these cancer cells.