中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
7期
901-903
,共3页
微管相关蛋白质类/生物合成%肿瘤抑制蛋白质类/生物合成%胃肿瘤/代谢
微管相關蛋白質類/生物閤成%腫瘤抑製蛋白質類/生物閤成%胃腫瘤/代謝
미관상관단백질류/생물합성%종류억제단백질류/생물합성%위종류/대사
Microtuhle-associated proteins/BI%Tumor suppressor proteios/BI%Stomach neoplasms/ME
目的 探讨Survivin、PTEN与胃癌的发生、发展、浸润转移及预后的关系.方法 运用原位杂交、免疫组化及TUNEL法检测Survivin、PTEN在胃癌中的表达以及细胞凋亡情况.结果 survivin在胃癌中的表达率明显高于癌旁正常胃组织(P<0.01);并且在临床分期较高、组织分化较差的胃癌中Survivin表达率较高(P<0.01,P<0.05).而PTEN在胃癌中的表达率明显低于癌旁正常胃组织(P<0.01);在临床分期较高、组织分化较差的胃癌中PTEN表达率较低(P<0.01).此外,胃癌中survivin与凋亡指数呈负相关(r=-0.861,P<0.01),PTEN与凋亡指数呈正相关(r=0.832,P<0.01).结论 Survivin可以作为早期胃癌检测及胃癌愈后判别的标志物之一;PTEN基因的失活可能促进了胃癌的发生、发展和浸润转移的进程.
目的 探討Survivin、PTEN與胃癌的髮生、髮展、浸潤轉移及預後的關繫.方法 運用原位雜交、免疫組化及TUNEL法檢測Survivin、PTEN在胃癌中的錶達以及細胞凋亡情況.結果 survivin在胃癌中的錶達率明顯高于癌徬正常胃組織(P<0.01);併且在臨床分期較高、組織分化較差的胃癌中Survivin錶達率較高(P<0.01,P<0.05).而PTEN在胃癌中的錶達率明顯低于癌徬正常胃組織(P<0.01);在臨床分期較高、組織分化較差的胃癌中PTEN錶達率較低(P<0.01).此外,胃癌中survivin與凋亡指數呈負相關(r=-0.861,P<0.01),PTEN與凋亡指數呈正相關(r=0.832,P<0.01).結論 Survivin可以作為早期胃癌檢測及胃癌愈後判彆的標誌物之一;PTEN基因的失活可能促進瞭胃癌的髮生、髮展和浸潤轉移的進程.
목적 탐토Survivin、PTEN여위암적발생、발전、침윤전이급예후적관계.방법 운용원위잡교、면역조화급TUNEL법검측Survivin、PTEN재위암중적표체이급세포조망정황.결과 survivin재위암중적표체솔명현고우암방정상위조직(P<0.01);병차재림상분기교고、조직분화교차적위암중Survivin표체솔교고(P<0.01,P<0.05).이PTEN재위암중적표체솔명현저우암방정상위조직(P<0.01);재림상분기교고、조직분화교차적위암중PTEN표체솔교저(P<0.01).차외,위암중survivin여조망지수정부상관(r=-0.861,P<0.01),PTEN여조망지수정정상관(r=0.832,P<0.01).결론 Survivin가이작위조기위암검측급위암유후판별적표지물지일;PTEN기인적실활가능촉진료위암적발생、발전화침윤전이적진정.
Objective To investigate the relationship among Survivin, PTEN and the occurrence, development, soakage and prog-nesis of gastric cancer. Methods The in-situ hybrization technique, immunochemistry and TUNEL method were used to detect the expres-sion of survivin mRNA, PTEN protein and apoptotic cell. Results The positive rate of survivin expression in gastric cancer was obvious higher than that in para-cancer tissue(P <0.01). Moreover, survivin expression rate in advanced clinical stage and low differentiation gastric cancer degree.was higher, compared with para-cancer tissue (P <0.01, P <0.05). The positive rate of PTEN expression in gas-tric cancer was obvious lower than that in pars-cancer tissue (P <0.01). The positive rate of PTEN expression in advanced clinical stage and low differentiation degree of tissue in gastric cancer was lower (P < 0.01). Furthermore, survivin and apoptesis indexes manifested negative relationship in gastric cancer(r =-0.861, P < 0.01), While PTEN and apoptosis indexes manifested positive relationship (r = 0.832, P < 0.01). Conclusions Survivin could be used as a novel marker to detect gastric cancer. PTEN gene lost function, which maybe promote the origin, growth, soakage and metastasis of gastric cancer.
