CAJ | 학술논문

目的分析白癜风患者皮损处Nrf2表达水平.方法采用AEC免疫组化法检测4例表皮移植的寻常型白癜风患者皮损处Nrf 2的表达,蛋白印迹分析表皮移植的8例寻常型白癜风患者皮损组织和其正常供皮处组织细胞核和细胞质中Nrf 2的表达水平,并进行统计学差异分析.结果与白癜风患者正常供皮处组织相比,皮损处Nrf 2的表达多数集中在角质形成细胞的胞质中,细胞核中表达量非常低.蛋白印迹结果表明,白癜风患者皮损处核蛋白中Nrf 2表达水平(0.10 ±0.03)显著低于正常部位(0.26 ±0.03,P＜0.01),胞质蛋白中皮损处Nrf 2表达水平(0.61±0.03)与正常部位(0.60 ±0.02)差异无统计学意义(P＞0.05).结论白癜风患者皮损处存在Nrf 2核转位异常.
목적 분석백전풍환자피손처Nrf2표체수평.방법 채용AEC면역조화법검측4례표피이식적심상형백전풍환자피손처Nrf 2적표체,단백인적분석표피이식적8례심상형백전풍환자피손조직화기정상공피처조직세포핵화세포질중Nrf 2적표체수평,병진행통계학차이분석.결과 여백전풍환자정상공피처조직상비,피손처Nrf 2적표체다수집중재각질형성세포적포질중,세포핵중표체량비상저.단백인적결과표명,백전풍환자피손처핵단백중Nrf 2표체수평(0.10 ±0.03)현저저우정상부위(0.26 ±0.03,P＜0.01),포질단백중피손처Nrf 2표체수평(0.61±0.03)여정상부위(0.60 ±0.02)차이무통계학의의(P＞0.05).결론 백전풍환자피손처존재Nrf 2핵전위이상.
Objective To investigate the level of NF-E2 related factor 2 (Nrf 2) in vitiligo lesions.Methods Tissue samples were obtained by press suction blisters at lesional and donor sites of 12 patients with vitiligo who were managed with epidermal transplantation. Four lesional samples from the patients were subjected to primary culture and the level of Nrf 2 was detected by AEC immunohistochemistry after 48hours of culture. Western blotting was utilized to further detect the level of cytoplasmic and nuclear Nrf 2 in tissue samples from the other 8 patients with vitiligo. Results Immunohistochemistry revealed that Nrf 2 was predominantly expressed in cytoplasm, rather than nuclei, of keratinocytes in vitiligo lesions compared with the normal skin of patients. The level of nuclear Nrf 2 was significantly lower in lesions than that in normal skin (0.10 ± 0.03 vs 0.26 ± 0.03, P ＜ 0.01) of the patients. In contrast, there was no significant dif- ference in the level of cytoplasmic Nrf2 between lesional and normal skin (0.61 ± 0.03 vs 0.60 ～ 0.02, P ＞0.05) of patients. Conclusion These results reveal an abnormality of nuclear translocation of Nrf 2 in vitili-go lesions.