中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
7期
914-916
,共3页
宇仁超%吕月霞%陈凤%谭丽娟
宇仁超%呂月霞%陳鳳%譚麗娟
우인초%려월하%진봉%담려연
髓样分化因子88/代谢%自身免疫疾病/病理生理学/血液%心肌炎/病理生理学/血液%心肌肌球蛋白%小鼠
髓樣分化因子88/代謝%自身免疫疾病/病理生理學/血液%心肌炎/病理生理學/血液%心肌肌毬蛋白%小鼠
수양분화인자88/대사%자신면역질병/병리생이학/혈액%심기염/병리생이학/혈액%심기기구단백%소서
Myeloid differentiation factor 88/metabolism%Autoimmune diseases/physiopathology/blood%Myocarditis/physiopathology/blood%Cardiac myosins%Mice
目的 通过对自发性免疫性心肌炎的Balb/c小鼠尾静脉注射MyD88高表达腺病毒来观测髓样分化因子88(Myeloid Differentiation Factor88,MyD88)对心肌炎症的影响.方法 将所购得的90只小鼠随机分为MyD88组、心肌炎组和对照组,各30只.MyD88组小鼠皮下注射心肌肌球蛋白混合液,3d后给予尾静脉注射MyD88高表达腺病毒混合液0.1ml；心肌炎组在相同部位注射等体积的心肌肌球蛋白混合液,3d后注射与实验组同剂量的PBS溶液；对照组仅皮下注射0.2ml的PBS.21 d后处死动物并制作切片,观测炎症浸润程度,并取眼球血测心肌坏死标志物cTnI及IgG、IgM抗体.结果 免疫后第21天,炎症评分心肌炎组显著高于对照组(2.39±1.23 vs 0.68±0.65,P＜0.05),表明自发性免疫性心肌炎小鼠建模成功.MyD88组炎症评分显著升高(F=94.194,P＜0.01),高于心肌炎组(3.56±1.34 vs 2.39±1.23,P＜0.05)和对照组(3.56±1.34 vs 0.68±0.65,P ＜0.01)；MyD88组小鼠血清cTnI、IgG和IgM检测值也明显高于心肌炎组和对照组(P＜0.01,P＜0.05).结论 MyD88参与了自发性免疫性心肌炎的发生发展.
目的 通過對自髮性免疫性心肌炎的Balb/c小鼠尾靜脈註射MyD88高錶達腺病毒來觀測髓樣分化因子88(Myeloid Differentiation Factor88,MyD88)對心肌炎癥的影響.方法 將所購得的90隻小鼠隨機分為MyD88組、心肌炎組和對照組,各30隻.MyD88組小鼠皮下註射心肌肌毬蛋白混閤液,3d後給予尾靜脈註射MyD88高錶達腺病毒混閤液0.1ml；心肌炎組在相同部位註射等體積的心肌肌毬蛋白混閤液,3d後註射與實驗組同劑量的PBS溶液；對照組僅皮下註射0.2ml的PBS.21 d後處死動物併製作切片,觀測炎癥浸潤程度,併取眼毬血測心肌壞死標誌物cTnI及IgG、IgM抗體.結果 免疫後第21天,炎癥評分心肌炎組顯著高于對照組(2.39±1.23 vs 0.68±0.65,P＜0.05),錶明自髮性免疫性心肌炎小鼠建模成功.MyD88組炎癥評分顯著升高(F=94.194,P＜0.01),高于心肌炎組(3.56±1.34 vs 2.39±1.23,P＜0.05)和對照組(3.56±1.34 vs 0.68±0.65,P ＜0.01)；MyD88組小鼠血清cTnI、IgG和IgM檢測值也明顯高于心肌炎組和對照組(P＜0.01,P＜0.05).結論 MyD88參與瞭自髮性免疫性心肌炎的髮生髮展.
목적 통과대자발성면역성심기염적Balb/c소서미정맥주사MyD88고표체선병독래관측수양분화인자88(Myeloid Differentiation Factor88,MyD88)대심기염증적영향.방법 장소구득적90지소서수궤분위MyD88조、심기염조화대조조,각30지.MyD88조소서피하주사심기기구단백혼합액,3d후급여미정맥주사MyD88고표체선병독혼합액0.1ml；심기염조재상동부위주사등체적적심기기구단백혼합액,3d후주사여실험조동제량적PBS용액；대조조부피하주사0.2ml적PBS.21 d후처사동물병제작절편,관측염증침윤정도,병취안구혈측심기배사표지물cTnI급IgG、IgM항체.결과 면역후제21천,염증평분심기염조현저고우대조조(2.39±1.23 vs 0.68±0.65,P＜0.05),표명자발성면역성심기염소서건모성공.MyD88조염증평분현저승고(F=94.194,P＜0.01),고우심기염조(3.56±1.34 vs 2.39±1.23,P＜0.05)화대조조(3.56±1.34 vs 0.68±0.65,P ＜0.01)；MyD88조소서혈청cTnI、IgG화IgM검측치야명현고우심기염조화대조조(P＜0.01,P＜0.05).결론 MyD88삼여료자발성면역성심기염적발생발전.
Objective To investigate the effect of myeloid differentiation factor88 (MyD88)on the experimental autoimmune myocarditis (EAM) in Balb/c mice.Methods Totally 90 Balb/c mice were randomly divided into three groups:MyD88 ( n =30),myocarditis ( n =30),and control ( n =30 )groups.An injection of MyD88 high expression adenovirus was carried out through vena caudalis at day 3.The mice of myocarditis group received porcine cardiac myosin at the same time and position.The mice of control group received PBS only.Serum and myocardium samples were gained at day 21.The inflammatory infiltration and the serum levels of cTnI,IgG,and IgM were examined.Results 21 days later,the inflammatory infiltration score of the myocarditis group was higher than the control group (2.39 ± 1.23 vs 0.68 ±0.65,P ＜0.05).The MyD88 group was significantly higher than the other 2 groups ( F =94.194,P ＜0.01 ),the myocarditis group ( 3.56 ± 1.34 vs 2.39 ± 1.23,P ＜ 0.05 ),and the control group ( 3.56 ±1.34 vs 0.68 ±0.65,P ＜0.01).The serum levels of cTnI,IgG,and IgM of the MyD88 group were significantly higher than the myocarditis group and the control group( P ＜0.01,P ＜0.05).Conclusions MyD88 participates in the pathogenesis of experimental autoimmune myocarditis in mice.
