中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2011年
8期
804-807
,共4页
金黄色葡萄球菌%甲氧西林耐药性金黄色葡萄球菌,社区分离型%SCCmec基因岛分型
金黃色葡萄毬菌%甲氧西林耐藥性金黃色葡萄毬菌,社區分離型%SCCmec基因島分型
금황색포도구균%갑양서림내약성금황색포도구균,사구분리형%SCCmec기인도분형
Staphylococcus aureus%Community acquired methicillin resistant Staphylococcus aureus%Staphylococcal cassette chromosome mec typing
目的 调查医科大学学生鼻腔中金黄色葡萄球菌(金葡菌)的定植状况,解析鼻腔定植社区型甲氧两林耐药性金葡菌(MRSA)克隆的分子生物学特点.方法 以无菌棉签从调查对象鼻腔中收集定植菌,鉴定出甲氧西林敏感性金葡菌(MSSA)和MRSA;多重PCR法分型MRSA携带的SCCmec基因岛;PCR测定pvl、seh、tsst1等毒素基因在菌株中的分布;脉冲场凝胶电泳(PFGE)检测MRSA菌株的脉冲场型.结果 2009年977名学生488份样本中鉴定出葡萄球菌,其中凝固酶阴性葡萄球菌(CoNS)和金葡菌分别为364份和124份,MRSA在金葡菌中所占比例为3.4%.2010年调查显示,657名学生中有310份样本鉴定为葡萄球菌属,其中CoNS为195份,金葡菌115份,MRSA在金葡菌中所占比例为7.7%.金葡菌在所有葡萄球菌属中所占比例为29.9%,而MRSA在金葡菌中所占比例为5.1%.MRSA菌株中共鉴定出5种SCCmec基因岛型,其中Ⅳa型最多(10株),为优势SCCmec基因岛类型.PFGE共有11种图谱型,7种(A～G)脉冲场型.10株MRSA均为pvl阳性.结论 1株起源于社区,基因型为Ⅳa SCCmec-pulsotype A且携带pvl毒素基因的特定MRSA克隆在健康大学生中传播.
目的 調查醫科大學學生鼻腔中金黃色葡萄毬菌(金葡菌)的定植狀況,解析鼻腔定植社區型甲氧兩林耐藥性金葡菌(MRSA)剋隆的分子生物學特點.方法 以無菌棉籤從調查對象鼻腔中收集定植菌,鑒定齣甲氧西林敏感性金葡菌(MSSA)和MRSA;多重PCR法分型MRSA攜帶的SCCmec基因島;PCR測定pvl、seh、tsst1等毒素基因在菌株中的分佈;脈遲場凝膠電泳(PFGE)檢測MRSA菌株的脈遲場型.結果 2009年977名學生488份樣本中鑒定齣葡萄毬菌,其中凝固酶陰性葡萄毬菌(CoNS)和金葡菌分彆為364份和124份,MRSA在金葡菌中所佔比例為3.4%.2010年調查顯示,657名學生中有310份樣本鑒定為葡萄毬菌屬,其中CoNS為195份,金葡菌115份,MRSA在金葡菌中所佔比例為7.7%.金葡菌在所有葡萄毬菌屬中所佔比例為29.9%,而MRSA在金葡菌中所佔比例為5.1%.MRSA菌株中共鑒定齣5種SCCmec基因島型,其中Ⅳa型最多(10株),為優勢SCCmec基因島類型.PFGE共有11種圖譜型,7種(A～G)脈遲場型.10株MRSA均為pvl暘性.結論 1株起源于社區,基因型為Ⅳa SCCmec-pulsotype A且攜帶pvl毒素基因的特定MRSA剋隆在健康大學生中傳播.
목적 조사의과대학학생비강중금황색포도구균(금포균)적정식상황,해석비강정식사구형갑양량림내약성금포균(MRSA)극륭적분자생물학특점.방법 이무균면첨종조사대상비강중수집정식균,감정출갑양서림민감성금포균(MSSA)화MRSA;다중PCR법분형MRSA휴대적SCCmec기인도;PCR측정pvl、seh、tsst1등독소기인재균주중적분포;맥충장응효전영(PFGE)검측MRSA균주적맥충장형.결과 2009년977명학생488빈양본중감정출포도구균,기중응고매음성포도구균(CoNS)화금포균분별위364빈화124빈,MRSA재금포균중소점비례위3.4%.2010년조사현시,657명학생중유310빈양본감정위포도구균속,기중CoNS위195빈,금포균115빈,MRSA재금포균중소점비례위7.7%.금포균재소유포도구균속중소점비례위29.9%,이MRSA재금포균중소점비례위5.1%.MRSA균주중공감정출5충SCCmec기인도형,기중Ⅳa형최다(10주),위우세SCCmec기인도류형.PFGE공유11충도보형,7충(A～G)맥충장형.10주MRSA균위pvl양성.결론 1주기원우사구,기인형위Ⅳa SCCmec-pulsotype A차휴대pvl독소기인적특정MRSA극륭재건강대학생중전파.
Objective To investigate the nasal colonization of Staphylococcus (S.) aureus strains among medical university students in Shenyang and to study the molecular epidemiological characteristics of methicillin resistant S. aureus (MRSA) strains. Methods Sterilized nasal swabs were used to collect nasal bacteria from both nares of the students. Nasal specimens were further identified as S. aureus strains, sensitive or resistant to methicillin through a series of tests. Molecular related methods including staphylococcal cassette chromosome mec (SCCmec) typing, pulsed- field gel electrophoresis (PFGE) , coagulase isotyping and minimum inhibitory concentration (MIC) determination etc. were used to characterize the isolates. Prevalence of the panton-valentine leukocidin (pvl) genes (lukS and F-PV) among the isolates was also assessed. Results Staphylococci were found in 488 specimens from 977 participants through the surveillance program, conducted in 2009. Of the 488 specimens being tested, 364 were identified as coagulase-negative staphylococci (CoNS) and 124 as S. aureus. MRSA strain among the S. aureus isolates was accounted for 3.4%. In the surveillance program conducted in 2010, staphylococci grew in 310 specimens fiom 657 participants. Of the 310 specimens tested, 195 were identified as CoNS and 115 as S. aureus. The percentage of MRSA strains among the S. aureus isolates was 7.7%. In total, 239 students carried S.aureus, and the percentage of MRSA carriers among the total specimens tested in this study was 5.1%.Most of the MRSA strains could be classified into one of the five types of SCCmec elements. Type Ⅳ a SCCmec strains were most frequent seen overall (10 isolates). A total of 11 pulsotypes were identified among the MRSA strains and were classified into 7 major groups (A to G) by the mutual correlations of their banding patterns. Ten MRSA strains were identified as pvl positive strains. Conclusion An MRSA clone (Ⅳ a SCCmec pulsotype A) carrying pvl toxin gene was found to be prevalent in the nares of the healthy university students.
