CAJ | 학술논문

目的观察柴胡疏肝汤对戊四氮致痫大鼠海马及额叶皮质c-fos表达的影响.方法选取经戊四氮诱导制作的癫痫模型大鼠48只,按随机数字表法分成6组:癫痫模型组、德巴金组、定痫丸组、柴胡疏肝汤低剂量组、柴胡疏肝汤中剂量组和柴胡疏肝汤高剂量组,每组各8只;另设正常对照组8只.正常对照组和癫痫模型组常规饲养,其他各组给予药物德巴金,定痫丸,低、中、高剂量柴胡疏肝汤处理,均连续灌胃治疗5周.免疫组化染色检测各组大鼠海马及额叶皮质c-fos的表达情况.结果戊四氮致痫后大鼠海马及额叶皮质c-fos表达明显增强,而应用中、高剂量的柴胡疏肝汤,德巴金和定痫丸治疗后,大鼠海马及额叶皮质c-fos表达均明显减弱,与癫痫模型组比较差异均有统计学意义(P<0.05),而柴胡疏肝汤低剂量组大鼠海马及额叶皮质c-fos表达无明显减弱.结论柴胡疏肝汤的抗癫痫作用机制可能与其含有柴胡皂甙及其他多种有效的抗癫痫成份多靶点干预海马及额叶皮质c-fos表达水平有关.
목적 관찰시호소간탕대무사담치간대서해마급액협피질c-fos표체적영향.방법 선취경무사담유도제작적전간모형대서48지,안수궤수자표법분성6조:전간모형조、덕파금조、정간환조、시호소간탕저제량조、시호소간탕중제량조화시호소간탕고제량조,매조각8지;령설정상대조조8지.정상대조조화전간모형조상규사양,기타각조급여약물덕파금,정간환,저、중、고제량시호소간탕처리,균련속관위치료5주.면역조화염색검측각조대서해마급액협피질c-fos적표체정황.결과 무사담치간후대서해마급액협피질c-fos표체명현증강,이응용중、고제량적시호소간탕,덕파금화정간환치료후,대서해마급액협피질c-fos표체균명현감약,여전간모형조비교차이균유통계학의의(P<0.05),이시호소간탕저제량조대서해마급액협피질c-fos표체무명현감약.결론 시호소간탕적항전간작용궤제가능여기함유시호조대급기타다충유효적항전간성빈다파점간예해마급액협피질c-fos표체수평유관.
Objective To observe the effect of chaihushugantang on the expression of c-fos in the hippocampus and frontal lobe cortex of pentylenetetrazole (PTZ)-induced epileptic rats. Methods Forty-eight PTZ-induced epileptic rats were randomly divided into 6 groups: epileptic model group,Valproate treatment group, Dingxianwan treatment group, and lower-dose, medium-dose and high-dose chaihushugantang treatment groups (n=8). Normal control group was also employed (n=8). The epileptic rats in the normal control group and epileptic model group were fed normally. Rats of the treatment groups were performed intragastric administration of medicines (Valproate, Dingxianwan and chaihushugantang) for 5 weeks in succession respectively. The expression of c-fos in the hippocampus and frontal lobe cortex of all the rats was observed. Results The expression of c-fos in the hippocampus and frontal lobe cortex of rats in the epileptic model group was significantly increased, while the c-fos expression in the hippocampus and the frontal lobe cortex of rats in the medium-dose and high-dose chaihushugantang treatment groups and Valproate treatment group and Dingxianwan treatment group was significantly decreased as compared with that in epileptic model group (P<0.05). But the c-fos expression in the hippocampus and the frontal lobe cortex of rats in the low-dose chaihushugantang treatment group showed no obvious decrease. Conclusion Chaihushugantang has good antiepileptic effect, might through affecting the c-fos expression in the epileptic rats. The antiepileptic mechanism of chaihushugantang can be related to saikosaponins and other antiepileptic constituents in it.