CAJ | 학술논문

目的建立肝癌姑息性切除术(PR)后可研究残癌生物学特性变化的转移性裸鼠人肝癌原位移植瘤模型.方法采用单肝叶双瘤源原位接种技术建立MHCC97H原位移植瘤模型,PR切除1瘤,观察5周.测量肿瘤大小,流式细胞术计数外周血循环肿瘤细胞数,荧光显微镜和苏木素-0伊红(HE)染色检测肝内、肺、腹腔转移等,免疫组织化学染色和Western blot检测脑和肿瘤组织Vimentin表达.结果双瘤源接种成瘤率95.6％、PR成功率95.3％、裸鼠5周总体存活率90.2％.假手术组、肝部分切除术组、PR组肿瘤大小、循环肿瘤细胞数均依次升高,肝内转移率分别为15.4％、41.7％、83.3％,肺转移率分别为61.5％、91.7％、100.0％,腹腔转移率分别为23.1％、50.0％、83.3％(P值均＜0.05).手术组脑和肿瘤组织Vimentin表达均显著上调.结论成功建立转移性裸鼠人肝癌原位移植瘤合并PR模型,可用于研究残癌生物学特性的变化.
목적 건립간암고식성절제술(PR)후가연구잔암생물학특성변화적전이성라서인간암원위이식류모형.방법 채용단간협쌍류원원위접충기술건립MHCC97H원위이식류모형,PR절제1류,관찰5주.측량종류대소,류식세포술계수외주혈순배종류세포수,형광현미경화소목소-0이홍(HE)염색검측간내、폐、복강전이등,면역조직화학염색화Western blot검측뇌화종류조직Vimentin표체.결과 쌍류원접충성류솔95.6％、PR성공솔95.3％、라서5주총체존활솔90.2％.가수술조、간부분절제술조、PR조종류대소、순배종류세포수균의차승고,간내전이솔분별위15.4％、41.7％、83.3％,폐전이솔분별위61.5％、91.7％、100.0％,복강전이솔분별위23.1％、50.0％、83.3％(P치균＜0.05).수술조뇌화종류조직Vimentin표체균현저상조.결론 성공건립전이성라서인간암원위이식류합병PR모형,가용우연구잔암생물학특성적변화.
Objective To construct a metastatic human hepatocellular carcinoma (HCC) orthotopic transplantation model with palliative resection (PR) in nude mice for investigating the biological characteristics of the residual cancer,and to verify its efficacy.Methods A metastatic human HCC orthotopic nude mice model (MHCC97H) was established by using the method of bi-tumor inoculation in a single liver lobe.Two weeks after transplantation,the PR was applied to resect one tumor from the two.lobes.The tumor size was measured five weeks postresection.The flow cytometry was used to count the circulating tumor cells (CTCs) in peripheral blood.The fluorescence microscope and hematoxylin and eosin (HE)staining were adopted to determine the metastasis of the liver,lung,or abdomen.The Vimentin expression in brain or tumor tissues was detected by using immunohistochemical staining or Western blotting respectively.Results The incidence of bi-tumor formation,successful PR,and 5-week overall survival were 95.6％,95.3％,and 90.2％ respectively.The tumor size and CTCs in sham operation group,liver partial hepatectomy group,and PR group were increased in gradient; the incidence of intrahepatic metastasis was 15.4％,41.7％ and 83.3％ ; lung metastasis rate was 61.5％,91.7％ and 100.0％ ; and abdomen metastasis rate was 23.1％,50.0％ and 83.3％,respectively (P＜0.05).The Vimentin expression in brain or tumor tissues was up-regulated after surgical resection.Conclusion A metastatic human HCC orthotopic nude mice model combined with PR is successfully constructed,and it may be used to investigate the biological characteristics of the residual cancer.