CAJ | 학술논문

目的探讨高压氧(HBO)对缺血再灌注小鼠脑组织中基质金属蛋白酶(MMPs)MMP-9、MMP-2 mRNA的表达及血脑屏障通透性的影响.方法复制清醒小鼠脑缺血再灌注模型,并于再灌注期间行0.25 MPa HBO治疗5次,在处死动物前1h经尾静脉注射2%伊文蓝(EB),采用逆转录多聚酶链反应(RT-PCR)方法及比色法分别检测脑组织中MMP-9、MMP-2 mRNA的表达及EB的含量.结果脑缺血再灌注组MMP-9、MMP-2 mRNA的表达及EB的含量明显高于假手术组,差异有统计学意义(P＜0.01),高压氧组与假手术组比较,脑组织中MMP-9、MMP-2 mRNA的表达及EB的含量相近(P＞0.05),HBO+脑缺血再灌注组脑组织中基质金属蛋白酶MMP-9、MMP-2 mRNA的表达及EB的含量明显低于脑缺血再灌注组,差异有统计学意义(P＜0.01).结论高压氧可明显减少脑缺血再灌注脑组织中基质金属白蛋酶MMP-9、MMP-2的表达,具有降低血脑屏障通透性的作用.
목적 탐토고압양(HBO)대결혈재관주소서뇌조직중기질금속단백매(MMPs)MMP-9、MMP-2 mRNA적표체급혈뇌병장통투성적영향.방법 복제청성소서뇌결혈재관주모형,병우재관주기간행0.25 MPa HBO치료5차,재처사동물전1h경미정맥주사2%이문람(EB),채용역전록다취매련반응(RT-PCR)방법급비색법분별검측뇌조직중MMP-9、MMP-2 mRNA적표체급EB적함량.결과 뇌결혈재관주조MMP-9、MMP-2 mRNA적표체급EB적함량명현고우가수술조,차이유통계학의의(P＜0.01),고압양조여가수술조비교,뇌조직중MMP-9、MMP-2 mRNA적표체급EB적함량상근(P＞0.05),HBO+뇌결혈재관주조뇌조직중기질금속단백매MMP-9、MMP-2 mRNA적표체급EB적함량명현저우뇌결혈재관주조,차이유통계학의의(P＜0.01).결론 고압양가명현감소뇌결혈재관주뇌조직중기질금속백단매MMP-9、MMP-2적표체,구유강저혈뇌병장통투성적작용.
Objective To investigate the effects of hyperbaric oxygen(HBO)on the expression of MMP-9 and MMP-2 mRNA in the brain tissues after cerebral ischemia and reperfusion,and the permeability of blood brain barrier(BBB).Methods Using cerebral ischemia-reperfusion models with conscious mice,0.25 MPa(atmosphaera absolutus,ATA)HBO was applied 5 times during the reperfusion period,and 2%Evan's blue(EB)was injected into the tail vein 1 hour before the animals were sacrificed.The expression of MMP-9 and MMP-2 mRNA and EB content were determined by RT-PCR and spectrophotometry.Results The expression of MMP-9 and MMP-2 mRNA aswell as EB content significantly increased in the ischemia-reperfusion group as compared with a sham surgery group.The expression of MMP-9 and MMP-2 mRNA,and EB levels in the HBO group were similar to those in the sham surgery group.The expression of MMP-9 and MMP-2 mRNA and EB levels in the group given HBO plus reperfusion group were significantly decreased as compared with those in the group receiving reperfusion alone. Conclusion HBO can significandy reduce the expression of MMP-9 and MMP-2 mRNA and the permeability of the BBB.