CAJ | 학술논문

包括血管内皮细胞在内的多种细胞和组织存在腺苷三磷酸双磷酸酶(apyrase), 但心内膜内皮细胞是否含有apyrase尚无报道.本文旨在研究牛心内膜内皮细胞apyrase的特性.以无机磷释放法检测培养牛心内膜内皮细胞(BEEC) apyrase的活性.公认的apyrase 抑制剂叠氮钠呈浓度依赖性地抑制apyrase活性; 另一种apyrase 抑制剂氟化钠 (20 mmol/L)也明显抑制apyrase活性. 而Na+/K+-ATPase的抑制剂哇巴因 (3 mmol/L)却不能抑制该酶活性. BEEC apyrase活性依赖于钙或镁等二价阳离子以及pH 的改变, EDTA或EGTA(1 mmol/L)均能完全抑制其活性, 在pH值为8.5时活性最高.由此可见, 牛心内膜内皮细胞存在叠氮钠敏感的、依赖于二价阳离子和pH值的腺苷三磷酸双磷酸酶活性.
포괄혈관내피세포재내적다충세포화조직존재선감삼린산쌍린산매(apyrase), 단심내막내피세포시부함유apyrase상무보도.본문지재연구우심내막내피세포apyrase적특성.이무궤린석방법검측배양우심내막내피세포(BEEC) apyrase적활성.공인적apyrase 억제제첩담납정농도의뢰성지억제apyrase활성; 령일충apyrase 억제제불화납 (20 mmol/L)야명현억제apyrase활성. 이Na+/K+-ATPase적억제제왜파인 (3 mmol/L)각불능억제해매활성. BEEC apyrase활성의뢰우개혹미등이개양리자이급pH 적개변, EDTA혹EGTA(1 mmol/L)균능완전억제기활성, 재pH치위8.5시활성최고.유차가견, 우심내막내피세포존재첩담납민감적、의뢰우이개양리자화pH치적선감삼린산쌍린산매활성.
Apyrase activities in some tissues and cells, such as peripheral vascular endothelial cells, have been reported, but these in endocardium endothelial cells have not been reported. The present study was to characterise the properties of bovine endocardium endothelial cells (BEEC)-associated apyrase. Apyrase activity was assayed by inorganic phosphate release, which could be inhibited concentration-dependently by NaN3, an apyrase inhibitor. NaF (20 mmol/L), another inhibitor of apyrase, also markedly inhibited the activity. EDTA or EGTA (1 mmol/L) could also inhibit the activity completely. However, the inhibitor for Na+/K+-ATPase, ouabain (3 mmol/L) did not affect the enzyme activity. BEEC apyrase activity was dependent on divalent cations (Ca2+ or Mg2+) and pH value.