农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
2期
63-66
,共4页
廖子荣%黄东益%牛杰%李俏%吴安迪
廖子榮%黃東益%牛傑%李俏%吳安迪
료자영%황동익%우걸%리초%오안적
Oryza sativa%Biomass%Mutant%Genetic analysis
[Objective] The study aimed to reveal the genetic model of a biomass mutant in Oryza sativa. [Method] ln the process of seresning and iden-tification of Bar-transgenic rice, a biomass mutant was found in 10 lines of TI progenies. The mutant was investigated for genetic analysis and agronomic traits by herbicide spraying and PCR amplification. [Result] The segregation ratio is consistent with mendelian law(3:1). The mutant assumed not only higher plant height, wider straw and earlier florescence, but also more tillers, bigger spikes and resuhantly higher biomass. PCR detections indicated that no co-segregation was observed between mutant traits and target gene(Bar) in the T-DNA inserted, proving that the mutant is not caused by the insertion of T-DNA containing target gene (Bar). [Conclusion] Our study may avail to understand the cloning of mutant gene and the mechanism of the mutant gene on biomass.
