CAJ | 학술논문

利用冷冻电镜显微技术和单颗粒三维重建方法获得BmCPV(家蚕质多角体);CSBV(中蜂囊状幼虫病痛毒);C6/36DNV(C6/36浓核病毒)的三维结构体数据,用空间低通滤波时体数据矢量场进行处理并进行三维显示,较原来的显示,提高了信噪比,增强了显示稳定性与显示质量.病毒每个表面细节和轴上突起更加清晰可见.
이용냉동전경현미기술화단과립삼유중건방법획득BmCPV(가잠질다각체);CSBV(중봉낭상유충병통독);C6/36DNV(C6/36농핵병독)적삼유결구체수거,용공간저통려파시체수거시량장진행처리병진행삼유현시,교원래적현시,제고료신조비,증강료현시은정성여현시질량.병독매개표면세절화축상돌기경가청석가견.
We present a novel method using a low pass filter to correct three-dimensional (3D) display in electron cryo-microscopy single particle reconstruction. SNR is increased after passing the filter. As a result, the methed has improvied both stability and performance of 3D display our method can be applied to process 1.3 nm(resolution) BmCPV (Bombyx mori Cytoplasmic Polyhedrosis Virus), 2.5 nm CSBV (Chinese Sacbrood Bee Virus), and 1.4 nm C6/36DNV(Bensonucleosis Virus) with the satisfied results.