CAJ | 학술논문

以水曲柳成熟种子为外植体材料,在不同增殖培养基中培养,应用间接酶联免疫法(ELISA)测定脱落酸(ABA)、吲哚乙酸(IAA)和玉米素核苷(ZR)3种内源激素,探讨不同细胞分裂素质量浓度和配比对水曲柳组培苗生长及内源激素的影响.研究结果表明:当年采收的水曲柳种子,消毒、浸泡处理后,播出种胚,可以解除水曲柳种子的休眠.增殖培养基中添加不同细胞分裂素都抑制了组培苗的高生长和生物量,但却可以解除组培苗顶端优势,诱导腋芽生长.与此同时,腋芽发生率与内源激素ABA、IAA和ZR质量分数不存在相关性,而与IAA/ZR比值存在负相关,但相关性并不显著.IAA/ZR比值的减少有可能有利于腋芽的诱导.
이수곡류성숙충자위외식체재료,재불동증식배양기중배양,응용간접매련면역법(ELISA)측정탈락산(ABA)、신타을산(IAA)화옥미소핵감(ZR)3충내원격소,탐토불동세포분렬소질량농도화배비대수곡류조배묘생장급내원격소적영향.연구결과표명:당년채수적수곡류충자,소독、침포처리후,파출충배,가이해제수곡류충자적휴면.증식배양기중첨가불동세포분렬소도억제료조배묘적고생장화생물량,단각가이해제조배묘정단우세,유도액아생장.여차동시,액아발생솔여내원격소ABA、IAA화ZR질량분수불존재상관성,이여IAA/ZR비치존재부상관,단상관성병불현저.IAA/ZR비치적감소유가능유리우액아적유도.
Seeds of Manchurian ash were sowed in culture media for propagation. The concentrations of IAA, ABA and ZR were determined by the immunoassay method. The effects of concentration and proportioning of the plant growth regulators on shoot growth and endogenous hormones were studied in the course of propagation of Manchurian ash. Results showed that the dormancy of the seeds harvested in the same year could be broken by immersing the seeds in sterile water after surface sterilization and peeling off the embryos. The height and biomass of the plantlets were inhibited when explants were cultured in media with different cytokinins, but the apical dominance was relieved and consequently axillary buds were induced. No correlations were found between induction rate of axillary buds and concentrations of ABA, IAA and ZR, while an insignificant negative correlation between induction rate of axillary buds and ratio of IAA/ZR was observed. The decrease of the ratio of IAA/ZR might be beneficial to the induction of axillary buds.