CAJ | 학술논문

以椰子油为原料利用固定化Candida antarctica sp.99-125脂肪酶转酯化制备了脂肪酸甲酯.分析了椰子油的脂肪酸组成,并测定了椰子油平均分子量,考查了关键因素对反应的影响及反应历程.实验结果表明:椰子油平均分子量为655.27;最优反应条件为:酶最小加入量为20%,水加入量为15%,正己烷加入量为4mL/2g椰子油.此反应条件下原料油转化率达到90%.
이야자유위원료이용고정화Candida antarctica sp.99-125지방매전지화제비료지방산갑지.분석료야자유적지방산조성,병측정료야자유평균분자량,고사료관건인소대반응적영향급반응역정.실험결과표명:야자유평균분자량위655.27;최우반응조건위:매최소가입량위20%,수가입량위15%,정기완가입량위4mL/2g야자유.차반응조건하원료유전화솔체도90%.
Fatty acid methyl esters have been prepared from coconut oil using immobilized Candida antarctica sp. 99-125 lipase. The fatty acid composition of the coconut oil was also analysed, The key factors influencing the transesterification reaction and the reaction mechanism were investigated. The average molecular weight of fatty acid methyl esters obtained was 655.27 and the optimum conditions were found to be: enzyme dosage of 20% (mass fraction based on oil); water dosage of 15% (mass fraction based on oil); hexane, 4 mL/2 g oil. A transesterifica-tion yield of 90% can be obtained under the optimum conditions.