CAJ | 학술논문

目的研究肝脏保留灌注溪黄草有效成分Nodosin对大鼠供肝缺血期的保护作用和作用机制.方法将实验雄性Wistar大鼠随机分为6组(n=3):空白对照组(乳酸钠林格液LR组)、实验组(低浓度NL组、中浓度NM组、高浓度NH组)和阳性对照组(Co组)、阴性对照组(Zn组).实验动物经门静脉灌注实验药物,于灌注后0、1、2 h时相点取肝脏组织标本,用比色法测定肝脏SOD酶活性和MDA含量,Banff标准评价损伤的病理表现,胆红素生成试验测定HO-1酶活性.结果 NH组和Co组肝脏保留灌注2 h后,MDA含量和Banff评分明显低于LR m(p<0.05),SOD酶活性和HO-1酶活性明显高于LR组(P<0.05);Zn组肝脏保留灌注2 h后.MDA含量明显高于LR组(P<0.05),SOD酶活性和HO-1酶活性明显低于LR组(P<0.05).结论保留灌注溪黄草有效成分Nodosin可以提高大鼠供肝抗氧化能力,减轻肝脏组织缺血期损伤,其作用可能是通过诱导肝脏组织HO-1酶表达上调来实现的.
목적 연구간장보류관주계황초유효성분Nodosin대대서공간결혈기적보호작용화작용궤제.방법 장실험웅성Wistar대서수궤분위6조(n=3):공백대조조(유산납림격액LR조)、실험조(저농도NL조、중농도NM조、고농도NH조)화양성대조조(Co조)、음성대조조(Zn조).실험동물경문정맥관주실험약물,우관주후0、1、2 h시상점취간장조직표본,용비색법측정간장SOD매활성화MDA함량,Banff표준평개손상적병리표현,담홍소생성시험측정HO-1매활성.결과 NH조화Co조간장보류관주2 h후,MDA함량화Banff평분명현저우LR m(p<0.05),SOD매활성화HO-1매활성명현고우LR조(P<0.05);Zn조간장보류관주2 h후.MDA함량명현고우LR조(P<0.05),SOD매활성화HO-1매활성명현저우LR조(P<0.05).결론 보류관주계황초유효성분Nodosin가이제고대서공간항양화능력,감경간장조직결혈기손상,기작용가능시통과유도간장조직HO-1매표체상조래실현적.
Objective To study the mechanism of protection from ischemic injury in rat liver by reserved infusing with Nodosin. Methods Eighteen male Wistar rats were randomly assigned into 6 groups (n = 3):Control group (lacta-ted ringer's solution, LR group) , experiment groups (Nodosin -Low concentration group, Nodosin -Middle concentration group, Nodosin - High concentration group),positive control group (Copp roup),and negative control group (Znpp group). Hepatic tissues were obtained Oh, 1h and 2h after perfusion. Activity of SOD and the quantity of MDA were assessed via using colorimetric method, while the severity of pathology injury was evaluated according to Banff Criteria, and the activity of HO - 1 was assessed via using bilirubin production test. Results The quantities of MDA and Banff's scores in NH group and positive control group (Copp group) were significantly lower than that in control group (P < 0.05) at 2 h, while activities of SOD and HO - 1 were significantly higher (P<0.05). The quantity of MDA in negative control group (Znpp group) was significantly higher than that in control group (P<0.05) at 2h, while the activities of SOD and HO - 1 was significantly higher (P < 0.05). Conclusion Reserved infusion with Nodosin can improve the anti - oxidant ability of rat liver to protect from hepatic ischemic injury, which may be mediated by induction of HO - 1 expression.