CAJ | 학술논문

目的研究同种异体骨髓间充质干细胞(MSCs)移植心肌梗死(MI)大鼠后缝隙连接蛋白43(Cx43)在不同时期的动态变化.方法建立大鼠MI模型.将同种异体MSCs用5-氮胞苷诱导成心肌样细胞并行荧光标记,经二次开胸注射入MI大鼠梗死区和梗死边缘区.各亚组分别于移植后4、8和12周在荧光显微镜下跟踪MSCs移植情况.同时用免疫组化分析Cx43表达与缝隙连接(GJ)分布.结果 MSCs体外诱导可分化为自发搏动的心肌样细胞,表达心肌特异性肌钙蛋白T(cTnT)和形成肌丝结构.MSCs移植后可长期存活并在4、8和12周,并有效上调缺血区Cx43的表达,改善GJ分布紊乱状态.在梗死区Cx43无特殊改变.结论 MSCs具有分化为心肌样细胞的可塑性,移植后上调MI后缺血区Cx43表达,改善GJ分布紊乱.
목적 연구동충이체골수간충질간세포(MSCs)이식심기경사(MI)대서후봉극련접단백43(Cx43)재불동시기적동태변화.방법 건립대서MI모형.장동충이체MSCs용5-담포감유도성심기양세포병행형광표기,경이차개흉주사입MI대서경사구화경사변연구.각아조분별우이식후4、8화12주재형광현미경하근종MSCs이식정황.동시용면역조화분석Cx43표체여봉극련접(GJ)분포.결과 MSCs체외유도가분화위자발박동적심기양세포,표체심기특이성기개단백T(cTnT)화형성기사결구.MSCs이식후가장기존활병재4、8화12주,병유효상조결혈구Cx43적표체,개선GJ분포문란상태.재경사구Cx43무특수개변.결론 MSCs구유분화위심기양세포적가소성,이식후상조MI후결혈구Cx43표체,개선GJ분포문란.
Objective To investigate the alterations of connexin 43 (Cx43) expression and its distribution at different stages of myocardial infarction (MI) in rats after transplantation of allogenic mesenchymal stem cells (MSCs).Methods Wistar rats were ligated on the left anterior descending coronary artery to make MI models.They were injected with allogenic MSCs,which were induced by 5-aza and labelled by DAPI,during the second operation after 7 days of MI.In subgroups,MSCs were detected by fluorescence microscope.Cx43 expression and GJ distribu-tion were examined by immunohistochemistry after 4,8 or 12 weeks respectively.Results MSCs differentiated into cardiac muscle cell-like cells which were capable of pulsing spontaneously,expressing cTnT and forming myofilament in vitro.Transplanted MSCs can survive in MI host and upregulate Cx43 expression and normalize Cx43 distribution at ischemic zones after 4,8 and 12w.No change of Cx43 was seen at infarcted zones.Conclusion MSCs have the plasticity of differentiating into cardiac muscle cell-like cells which can continuously upregulate Cx43 expression and normalize Cx43 distribution at ischemic zones after 4,8 and 12w.