CAJ | 학술논문

目的研究人工冬虫夏草对香烟烟雾所致慢性阻塞性肺疾病(COPD)大鼠模型的治疗和作用机制.方法 SD健康雄性大鼠随机分为对照组、COPD组、造模前给药组、造模后给药组、中剂量(2.5 g·kg-1·d-1)和高剂量(5 g·kg-1·d-1)治疗组共6组,每组10只.动物连续烟熏90 d造模.造模前给药组于造模前两周开始给药,治疗组在造模前给药的基础上持续给药直至造模结束.造模后给药组为在造模结束后给药两周.测定各组大鼠肺功能,观察肺组织病理学变化,提取肺组织总蛋白进行双向电泳,对表达差异显著的蛋白点进行鉴定分析.结果气道阻力均值COPD组(0.386 9 cm H2O·ml-1·s)明显高于对照组(0.263 0 cm H2O·ml-1·s)(P ＜0.05);造模前给药组(0.252 5 cm H2O·ml-1·s)、中剂量治疗组(0.223 3 cm H2O·ml-1·s)、高剂量治疗组(0.184 6cm H2O· ml-1·s)、造模后给药组(0.301 1 cm H2O·ml-1·s)均明显低于COPD组(P＜0.05),与对照组相比差异无统计学意义.给药各组特别是高剂量组与COPD组相比支气管和肺部病理情况均有明显改善,结果差异有统计学意义(P＜0.05).与对照组相比有14种蛋白质的表达量在COPD组中显著下降.谷胱甘肽过氧化物酶、醛脱氢酶及线粒体醛脱氢酶前体等三种蛋白则在高剂量治疗组中表达量得到恢复和改善.结论人工冬虫夏草对大鼠COPD有明确的治疗作用,推测与抑制或改善肺部组织氧化与抗氧化失衡以及改善全身状况有关.
목적 연구인공동충하초대향연연무소치만성조새성폐질병(COPD)대서모형적치료화작용궤제.방법 SD건강웅성대서수궤분위대조조、COPD조、조모전급약조、조모후급약조、중제량(2.5 g·kg-1·d-1)화고제량(5 g·kg-1·d-1)치료조공6조,매조10지.동물련속연훈90 d조모.조모전급약조우조모전량주개시급약,치료조재조모전급약적기출상지속급약직지조모결속.조모후급약조위재조모결속후급약량주.측정각조대서폐공능,관찰폐조직병이학변화,제취폐조직총단백진행쌍향전영,대표체차이현저적단백점진행감정분석.결과 기도조력균치COPD조(0.386 9 cm H2O·ml-1·s)명현고우대조조(0.263 0 cm H2O·ml-1·s)(P ＜0.05);조모전급약조(0.252 5 cm H2O·ml-1·s)、중제량치료조(0.223 3 cm H2O·ml-1·s)、고제량치료조(0.184 6cm H2O· ml-1·s)、조모후급약조(0.301 1 cm H2O·ml-1·s)균명현저우COPD조(P＜0.05),여대조조상비차이무통계학의의.급약각조특별시고제량조여COPD조상비지기관화폐부병리정황균유명현개선,결과차이유통계학의의(P＜0.05).여대조조상비유14충단백질적표체량재COPD조중현저하강.곡광감태과양화물매、철탈경매급선립체철탈경매전체등삼충단백칙재고제량치료조중표체량득도회복화개선.결론 인공동충하초대대서COPD유명학적치료작용,추측여억제혹개선폐부조직양화여항양화실형이급개선전신상황유관.
Objective To investigate the therapeutic effect of cultivated Cordyceps sinensis on rats' chronic obstructive pulmonary disease (COPD) by inhaling cigarette and analyze on the mechanism.Methods Ten-week-old male rats were selected and divided randomly into 6 groups as control group,COPD group,pre-administrated group,post-administrated group,moderate dosage group and high dosage group.Each group has 10 animals.The experimental rats were fumigated with simple smoking exposure for 90 days to establish a COPD model.Pre-administrated group were treated with Cordyceps sinensis for two weeks before fumigation.Besides receiving two weeks' drug before fumigation,moderate dosage (2.5 g · kg-1 · d-1 ) and high dosage (5 g · kg-1 · d-1 ) were given drug for the whole fumigation period.Post-administrated.group received Cordyceps sinensis for two weeks after fumigation.The animals' pulmonary function and lung tissue pathological changes were examined and observed.The total proteins from lung tissue were collected and separated by 2-dimensional electrophosis.The different significant expression proteins were identified by MS and analyzed with NCBI data.Results The average value of airway resistance of COPD group (0.386 9 cm H2O · ml-1 · s) is higher significantly than that of control group (0.263 0 cm H2O · ml-1 · s) ( P ＜0.05).The values from pre-administrated group (0.252 5 cm H2O · ml-1 · s),moderate dosage group (0.223 3 cm H2O · ml-1 · s),high dosage group (0.184 6 cm H2O · ml-1 · s) and post-administrated group (0.301 1 cm H2O · ml-1 · s),is lower significantly than that of COPD group (all P ＜0.05).But the values from the 4 drug-treatment groups have no statistical difference when compared with that of the control group.Statistically,the pulmonary functions and pathological conditions of the treatment groups,especially high dosage group,were improved greatly when compared with that of COPD group were improved significantly.There are 14 lung tissue proteins from COPD group showing a lower expression compared with that of control group.Glutathione peroxidase,aldehyde dehydrogenase and mitochondrial aldehyde dehydrogenase precursor were returned to normal expression in high dosage group.Conclusions The cultivated Cordyceps sinensis showed a evident therapeutic effect on rats' COPD,and the mechanism might be related to inhibiting and improving the imbalance of oxidation and antioxidationin in lung tissues and modulating and improving whole body condition.