CAJ | 학술논문

目的探讨RNA干扰树突细胞(Dc)组织相容性复合物1(MHC-1)表达后获得的CD8+CD28-抑制性T细胞1(Ts)对小肠移植免疫耐受的的影响.方法通过siRNA干扰DC MHC-Ⅰ表达后,诱导获得CD8+CD28-Ts.建立南Wistar大鼠移植到SD大鼠的小肠移植模型36例,随机分为A组(转染实验组)、B组(未转染组,注射普通T细胞)和C组(移植对照组.注射生理盐水).术后14 d,每组各随机挑选6例,取移植大鼠小肠和血液标本行移植小肠组织病理学检查,并检测移植大鼠血清TGF- β、IFN-γ水平及回肠黏膜Na+-K+-ATP酶活性,观察移植大鼠的存活时间.结果术后第14天,A组移植大鼠血清中TGF-β和IFN-γ表达水平高于B、C组(P<0.05).A组大鼠肠黏膜Na+-K+-ATP酶活性为(6.3±1.0)kU/g,明显高于B组的(3.6±0.9)kU/g和C组的(2.9±1.3)kU/g(P<0.05).A组移植小肠病理Parks评分分级明显低于B组和c组(P<0.05).A、B和C组移植后大鼠中位生存时间分别为32.0、17.5和21.0 d,A组存活时间明显优于B组和c组(P<0.05).结论将RNA干扰DC MHC-Ⅰ表达所获得的CD8+CD28-Ts过继小肠移植大鼠.可以减轻移植大鼠的损伤程度.抑制免疫排斥反应.
목적 탐토RNA간우수돌세포(Dc)조직상용성복합물1(MHC-1)표체후획득적CD8+CD28-억제성T세포1(Ts)대소장이식면역내수적적영향.방법 통과siRNA간우DC MHC-Ⅰ표체후,유도획득CD8+CD28-Ts.건립남Wistar대서이식도SD대서적소장이식모형36례,수궤분위A조(전염실험조)、B조(미전염조,주사보통T세포)화C조(이식대조조.주사생리염수).술후14 d,매조각수궤도선6례,취이식대서소장화혈액표본행이식소장조직병이학검사,병검측이식대서혈청TGF- β、IFN-γ수평급회장점막Na+-K+-ATP매활성,관찰이식대서적존활시간.결과 술후제14천,A조이식대서혈청중TGF-β화IFN-γ표체수평고우B、C조(P<0.05).A조대서장점막Na+-K+-ATP매활성위(6.3±1.0)kU/g,명현고우B조적(3.6±0.9)kU/g화C조적(2.9±1.3)kU/g(P<0.05).A조이식소장병리Parks평분분급명현저우B조화c조(P<0.05).A、B화C조이식후대서중위생존시간분별위32.0、17.5화21.0 d,A조존활시간명현우우B조화c조(P<0.05).결론 장RNA간우DC MHC-Ⅰ표체소획득적CD8+CD28-Ts과계소장이식대서.가이감경이식대서적손상정도.억제면역배척반응.
Objective To investigate the effect of CD8+CD28- suppressor T cells (%) induced by dendritic cell (DC) with major histocompatibility complex 1 (MHC-1) expression RNA interference on immune tolerance in rat intestinal transplantation. Methods The expression level of CD8+CD28- Ts were successfully induced by DC with MHC-1 expression interfered by RNA interference technique under the stimulator of allograft antigen. Orthotopic intestinal transplantation was performed in 36 rats by modified three cuffs method. The recipients were randomly divided into three groups(12 rats in each group) :group A was experimental group with CD8+CD28- Ts being administrated, mixed T cells were injected in group B, while in group C, NS were administrated. On the first day and the seventh day posttransplant, the 36 rats of the 3 groups were administrated through vena dorsalis penis respectively.Six rats were selected randomly from each group and the animals were sacrificed on the 14 th day postoperatively, serum levels of TGF-β, IFN-γ and the values of Na+-K+-ATPase activity of the intestinal graft were assayed and the intestinal pathologie morphology, intestinal allograft survival were observed concerning the remainders. Results On the 14 th day after operation, the expression levels of TGF-β and IFN-γ in group A were significantly up-regulated as compared with those in group B and group C(P<0.05). Na+-K+-ATPase activity in group A was(6.3±1.0) kU/g, much higher than the levels of group B(3.6±0.9)kU/g and group C(2.9±1.3) kU/g and the differences were significant(P<0.05).The data suggested preliminarily that pathological scores of intestinal graft in group A were lower than those in group B and group C. The survival time of the recipients in group A was 32.0 days, much longer than that in group B (17.5 days, P<0.05) and group C(21.0 days, P<0.05). Conclusion CDS+CD28-Ts indueed by DC with MHC-1 expression RNA interference can alleviate acute rejection and lead to immune tolerance in rat intestinal transplantation.