中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2010年
24期
3351-3353,后插1
,共4页
咪达唑仑%固相萃取%1'-羟基咪达唑仑%4-羟基咪达唑仑%血药浓度
咪達唑崙%固相萃取%1'-羥基咪達唑崙%4-羥基咪達唑崙%血藥濃度
미체서륜%고상췌취%1'-간기미체서륜%4-간기미체서륜%혈약농도
Midazolam%Solid-phase extraction%1 '-hydroxymidazolam%4-hydroxymidazolam%Plasma concentration
目的 建立测定人血浆中咪达唑仑及其代谢产物浓度的固相萃取和HPLC方法.方法 采用HPLC法分别测定29例ICU机械通气患儿给药24 h后血浆中咪达唑仑及其代谢产物浓度.色谱条件为:ZORBAX Eclipse XDB-C18色谱柱(4.6×250 mm,5 μm);流动相为7.56 mmol/L硫酸铵溶液-乙腈,梯度洗脱,柱温25 ℃,流速1.0 ml/mmin,紫外检测波长254 nm.结果 4-羟基咪达唑仑、1'-羟基咪达唑仑和咪达唑仑的保留时间分别为8.14、9.09和11.18 min.咪达唑仑及其羟基代谢产物的相对回收率98.88%～100.01%,RSD＜4%.29例患者血浆中咪达唑仑、1'-羟基咪达唑仑和4-羟基咪达唑仑的平均浓度为0.68,0.28和0.08mg/L.结论 该方法适用于咪达唑仑及其代谢产物血药浓度的常规监测.
目的 建立測定人血漿中咪達唑崙及其代謝產物濃度的固相萃取和HPLC方法.方法 採用HPLC法分彆測定29例ICU機械通氣患兒給藥24 h後血漿中咪達唑崙及其代謝產物濃度.色譜條件為:ZORBAX Eclipse XDB-C18色譜柱(4.6×250 mm,5 μm);流動相為7.56 mmol/L硫痠銨溶液-乙腈,梯度洗脫,柱溫25 ℃,流速1.0 ml/mmin,紫外檢測波長254 nm.結果 4-羥基咪達唑崙、1'-羥基咪達唑崙和咪達唑崙的保留時間分彆為8.14、9.09和11.18 min.咪達唑崙及其羥基代謝產物的相對迴收率98.88%～100.01%,RSD＜4%.29例患者血漿中咪達唑崙、1'-羥基咪達唑崙和4-羥基咪達唑崙的平均濃度為0.68,0.28和0.08mg/L.結論 該方法適用于咪達唑崙及其代謝產物血藥濃度的常規鑑測.
목적 건립측정인혈장중미체서륜급기대사산물농도적고상췌취화HPLC방법.방법 채용HPLC법분별측정29례ICU궤계통기환인급약24 h후혈장중미체서륜급기대사산물농도.색보조건위:ZORBAX Eclipse XDB-C18색보주(4.6×250 mm,5 μm);류동상위7.56 mmol/L류산안용액-을정,제도세탈,주온25 ℃,류속1.0 ml/mmin,자외검측파장254 nm.결과 4-간기미체서륜、1'-간기미체서륜화미체서륜적보류시간분별위8.14、9.09화11.18 min.미체서륜급기간기대사산물적상대회수솔98.88%～100.01%,RSD＜4%.29례환자혈장중미체서륜、1'-간기미체서륜화4-간기미체서륜적평균농도위0.68,0.28화0.08mg/L.결론 해방법괄용우미체서륜급기대사산물혈약농도적상규감측.
Objective To develop a solid-phase extraction and HPLC method for the determination of midazolam and its hydroxy metabolites in child plasma. Methods Blood plasma samples were collected twenty four hours after treated with midazolam by continuous infusion of 2μg·kg-1·min-1 in our intensive care unit, the concentration of midazolam and its metabolites in plasma was detected by solid-phase extraction and RP-HPLC. The separation was performed on ZORBAX Eclipse XDB～C18 column(4.6 ×250mm,5μ m) with 7.56mmol/L ammonium sulfate - acetonitrile as mobile phase. The flow rate was 1.0ml/min,the column temperature was 25℃ and the wavelength of UV detector was 254nm. Results The retention time of 4-hydroxymidazolam, 1'-hydroxymidazolam and midazolam was detected as 8.14,9.09 and 11.18 min. The average recoveries for midazolam and its metabolites was 98.88% ～100.01% ,and RSD ＜ 4%. 29 patients took part in the study, the average plasma concentration of midazolam, 1'-hydroxymidazolam and 4-hydroxymidazolam was 0.68,0.28 and 0.08mg/L. Conclusion It is suitable for convention monitoring of the midazolam and its hydroxy metabolites concentration in the plasma
