中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2008年
12期
1629-1631
,共3页
钟喆%袁贤瑞%何正文%任年军%高洪波%章凯%纪春晓
鐘喆%袁賢瑞%何正文%任年軍%高洪波%章凱%紀春曉
종철%원현서%하정문%임년군%고홍파%장개%기춘효
干扰素Ⅱ型%星形细胞瘤%HLA抗原
榦擾素Ⅱ型%星形細胞瘤%HLA抗原
간우소Ⅱ형%성형세포류%HLA항원
Interferon type Ⅱ%Astrocytoma%HLA antigens
目的 在细胞学层次上研究γ-IFN对星形细胞肿瘤HLA-G mRNA表达的影响.方法 原代细胞培养成功的基础上,用不同浓度γ-IFN处理,应用RT-PCR检测HLA-G mRNA表达水平的变化.结果γ-IFN处理后,HLA-G基因表达阴性的肿瘤细胞仍未见HLA-G mRNA的表达;不同浓度γ-IFN均可上调HLA-G基因表达阳性肿瘤细胞的HLA-G mRNA水平,并呈刑量依赖关系.结论 γ-IFN可以上调HLA-G基因阳性表达肿瘤细胞的HLA-G基因表达.
目的 在細胞學層次上研究γ-IFN對星形細胞腫瘤HLA-G mRNA錶達的影響.方法 原代細胞培養成功的基礎上,用不同濃度γ-IFN處理,應用RT-PCR檢測HLA-G mRNA錶達水平的變化.結果γ-IFN處理後,HLA-G基因錶達陰性的腫瘤細胞仍未見HLA-G mRNA的錶達;不同濃度γ-IFN均可上調HLA-G基因錶達暘性腫瘤細胞的HLA-G mRNA水平,併呈刑量依賴關繫.結論 γ-IFN可以上調HLA-G基因暘性錶達腫瘤細胞的HLA-G基因錶達.
목적 재세포학층차상연구γ-IFN대성형세포종류HLA-G mRNA표체적영향.방법 원대세포배양성공적기출상,용불동농도γ-IFN처리,응용RT-PCR검측HLA-G mRNA표체수평적변화.결과γ-IFN처리후,HLA-G기인표체음성적종류세포잉미견HLA-G mRNA적표체;불동농도γ-IFN균가상조HLA-G기인표체양성종류세포적HLA-G mRNA수평,병정형량의뢰관계.결론 γ-IFN가이상조HLA-G기인양성표체종류세포적HLA-G기인표체.
Objective To study the effects of γ-IFN on the expression of HLA-G mRNA in primary cultured astrocytoma cells. Methods Different concentrations of γ-IFN were added to primary cultured cells, and HLA-G mRNA were detected by RT-PCR. Results After γ-IFN treatment, HLA-G mRNA can not be determined from HLA-G originally negative astrocytoma cells. The expression of HLA-G are up - regulated in all original HLA-G positive astrocytoma cells in a dose-dependent manner. Conclusion γ-IFN can increase the ex-pression of HLA-G gene in the primary cultured astrocytoma cells which HLA - G are originally positive.