CAJ | 학술논문

目的探讨严重烧伤大鼠肝脏内质网应激相关蛋白表达变化及其可能意义.方法 Wistar大鼠64只随机数字表法随机分为对照组及烧伤组(30％体表面积Ⅲ.烫伤),各32只.伤后第1、4、7、14天采集大鼠肝脏组织.用Western印迹法检测内质网应激通路中葡萄糖调节蛋白78(GRP78)、C/EBP同源蛋白(CHOP)、半胱氨酸天冬氨酸蛋白酶(caspase)12及caspase-3活性体蛋白表达.用原位末端转移酶标记(TUNEL)法检测肝脏细胞凋亡率.结果与对照组相比,伤后第1、4、7、14天大鼠肝脏GRP78表达均显著增高(分别为1.29±0.11比1.00±0.00、1.28±0.12比0.95±0.16、1.29±0.14比0.93±0.06、1.41 ±0.17比1.02 ±0.13)；伤后第1、4天CHOP表达显著增高(分别为1.72±0.07比1.00±0.00、1.82±0.18比1.46±0.08)；而伤后第1、4、7天caspase-12活性体和caspase-3活性体表达显著增高(分别为2.05±0.65比1.00 ±0.00、2.16±0.69比0.95±0.21、1.98±0.56比0.90±0.22和1.96±0.15比1.00±0.00、1.40±0.14比1.07±0.12、1.77±0.17比1.15±0.21)；伤后第1、4、7、14天肝脏细胞凋亡率(％)均显著升高(分别为27.20±3.63比5.00±0.71、16.40±1.52比5.40±1.14、27.60±1.82比7.40±1.14、10.20±1.92比5.20±1.64)；以上差异均有统计学意义(均P ＜0.05).结论严重烧伤后大鼠肝脏内质网应激反应激活,相关蛋白GRP78、CHOP、caspase-12及caspase-3活性体表达增加.
목적 탐토엄중소상대서간장내질망응격상관단백표체변화급기가능의의.방법 Wistar대서64지수궤수자표법수궤분위대조조급소상조(30％체표면적Ⅲ.탕상),각32지.상후제1、4、7、14천채집대서간장조직.용Western인적법검측내질망응격통로중포도당조절단백78(GRP78)、C/EBP동원단백(CHOP)、반광안산천동안산단백매(caspase)12급caspase-3활성체단백표체.용원위말단전이매표기(TUNEL)법검측간장세포조망솔.결과 여대조조상비,상후제1、4、7、14천대서간장GRP78표체균현저증고(분별위1.29±0.11비1.00±0.00、1.28±0.12비0.95±0.16、1.29±0.14비0.93±0.06、1.41 ±0.17비1.02 ±0.13)；상후제1、4천CHOP표체현저증고(분별위1.72±0.07비1.00±0.00、1.82±0.18비1.46±0.08)；이상후제1、4、7천caspase-12활성체화caspase-3활성체표체현저증고(분별위2.05±0.65비1.00 ±0.00、2.16±0.69비0.95±0.21、1.98±0.56비0.90±0.22화1.96±0.15비1.00±0.00、1.40±0.14비1.07±0.12、1.77±0.17비1.15±0.21)；상후제1、4、7、14천간장세포조망솔(％)균현저승고(분별위27.20±3.63비5.00±0.71、16.40±1.52비5.40±1.14、27.60±1.82비7.40±1.14、10.20±1.92비5.20±1.64)；이상차이균유통계학의의(균P ＜0.05).결론 엄중소상후대서간장내질망응격반응격활,상관단백GRP78、CHOP、caspase-12급caspase-3활성체표체증가.
Objective To explore the expression of endoplasmic reticulum stress (ERS) associated proteins in livers of severely burned rats and examine its potential significance.Methods Sixty-four Wistar rats were randomly divided into the control and burn groups (30％ total body surface area full-thickness thermal injury) (n =32 each).Livers were harvested at Day 1,4,7,14 post-burn.Western blot was used to detect the expressions of endoplasmic reticulum stress associated proteins glucose regulated protein 78 ( GRP78 ),C/EBP-homologous protein ( CHOP ),active caspase-12 and active caspase-3. Hepatic apoptosis was assessed by the assay of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL).Results Compared with the control group,the expression of GRP78 became elevated at Day 1,4,7,14 post-burn (1.29 ±0.11 vs 1.00 ±0.00,1.28 ±0.12 vs 0.95 ±0.16,1.29 ±0.14 vs 0.93 ±0.06,1.41 ±0.17 vs 1.02 ±0.13 respectively) ; the expression of CHOP was higher at Day 1,4 ( 1.72 ±0.07 vs 1.00 ±0.00,1.82 ±0.18 vs 1.46 ±0.08 respectively) while active caspase-12 and active caspase-3 increased at Day 1,4,7 post-burn (2.05 ±0.65 vs 1.00 ±0.00,2.16±0.69 vs 0.95 ±0.21,1.98 ±0.56 vs 0.90 ±0.22; 1.96±0.15 vs 1.00 ±0.00,1.40±0.14 vs 1.07 ± 0.12,1.77 ±0.17 vs 1.15 ±0.21 respectively) ; the apoptotic index (％) of hepatocytes was higher at Day 1,4,7,14 post-burn (27.20±3.63 vs 5.00 ±0.71,16.40±1.52 vs 5.40 ±1.14,27.60±1.82 vs 7.40 ±1.14,10.20±1.92 vs 5.20 ± 1.64 respectively).All results were statistically significant ( all P ＜ 0.05).Conclusion ERS activates and expressions of associated proteins GRP78,CHOP,active caspase-12 and active caspase-3 increase in livers of severely burned rats.