CAJ | 학술논문

目的探讨海洋蛋白肽(marine protein peptide,MPP)对小鼠的免疫调节作用及其可能的机制.方法以小鼠为动物模型,观察不同剂量(0.22 g/kg、0.45 g/kg和1.35 g/kg)MPP对小鼠体重、免疫器官相对重量、细胞免疫功能、体液免疫功能、单核巨噬细胞功能、NK细胞活性、脾脏T淋巴细胞亚群以及血清中细胞因子的影响.结果 MPP 0.22 g/kg剂量组Con A诱导的小鼠淋巴细胞增殖能力(0.33±0.21)和足跖肿胀度(0.36±0.11)mm,较对照组的相应两值(0.15±0.10)和(0.21±0.10)mm均有显著提高.溶血空斑数对数转换值在MPP 0.22 g/kg组(1.64±0.06)、0.45 g/kg组(1.59±0.05)和1.35 g/kg组(1.56±0.10)中均显著高于对照组(1.38±0.10);血清半数溶血值在MPP 0.22 g/kg(141.00±23.00)和0.45 g/kg组(130.40±33.20)中均显著高于对照组(100.30±19.40).MPP 0.22/kg组的NK细胞活性对数转换值(1.672±0.142)比对照组(1.392±0.182)明显升高.CD4+T辅助细胞(Th细胞)百分比在MPP 0.22 g/kg组(32.84±3.776)%和0.45 g/kg组(32.42±3.507)%中均显著高于对照组(25.06±0.354)%.血清IL-2浓度在MPP 0.22 g/kg组181.06 pg/ml、0.45 g/kg组94.84 pg/ml和1.35 g/kg 102.61 pg/ml中均显著高于对照组0.50 pg/ml;血清IL-5浓度在MPP 0.22 g/kg组38.31 pg/ml中显著高于对照组0.50 pg/ml.结论 MPP具有增强小鼠免疫功能的作用,可能通过增强Th细胞功能以及刺激细胞因子分泌而实现.
목적 탐토해양단백태(marine protein peptide,MPP)대소서적면역조절작용급기가능적궤제.방법 이소서위동물모형,관찰불동제량(0.22 g/kg、0.45 g/kg화1.35 g/kg)MPP대소서체중、면역기관상대중량、세포면역공능、체액면역공능、단핵거서세포공능、NK세포활성、비장T림파세포아군이급혈청중세포인자적영향.결과 MPP 0.22 g/kg제량조Con A유도적소서림파세포증식능력(0.33±0.21)화족척종창도(0.36±0.11)mm,교대조조적상응량치(0.15±0.10)화(0.21±0.10)mm균유현저제고.용혈공반수대수전환치재MPP 0.22 g/kg조(1.64±0.06)、0.45 g/kg조(1.59±0.05)화1.35 g/kg조(1.56±0.10)중균현저고우대조조(1.38±0.10);혈청반수용혈치재MPP 0.22 g/kg(141.00±23.00)화0.45 g/kg조(130.40±33.20)중균현저고우대조조(100.30±19.40).MPP 0.22/kg조적NK세포활성대수전환치(1.672±0.142)비대조조(1.392±0.182)명현승고.CD4+T보조세포(Th세포)백분비재MPP 0.22 g/kg조(32.84±3.776)%화0.45 g/kg조(32.42±3.507)%중균현저고우대조조(25.06±0.354)%.혈청IL-2농도재MPP 0.22 g/kg조181.06 pg/ml、0.45 g/kg조94.84 pg/ml화1.35 g/kg 102.61 pg/ml중균현저고우대조조0.50 pg/ml;혈청IL-5농도재MPP 0.22 g/kg조38.31 pg/ml중현저고우대조조0.50 pg/ml.결론 MPP구유증강소서면역공능적작용,가능통과증강Th세포공능이급자격세포인자분비이실현.
Objective To observe the effects of marine protein peptide(MPP)on immunnomodulating in mice and its possible mechanism.Methods Female ICR mice(6-8 weeks old)were administered the MPP for 4 weeks with the dose of 0.22 g/kgBW、0.45 g/kgBW and 1.35 g/kgBW.Spleen and thymus were weighted and cell-mediated immune functions,humoral immune functions,phagocytic functions of mononuclear phagocyte,NK cell activity assays,the T cell subpopulation of the spleen tissue by the flow cvtometer and the concentrations of cytokines in serum by cytometric bead array were examined.Results The capacity of lymphocyte proliferation induced by Con A(0.33±0.21),DTH response(0.36±0.11)mm in MPP 0.22 g/kgBW group were significantly increased in comparison with these values in control group(0.15±0.10)and(0.21±0.10)mm,respectively,P<0.05. IgM-PFC number of MPP 0.22g/kgBW group(1.64±0.06)、0.45 g/kgBW group(1.59±0.05)and 1.35 g/kgBW group(1.56±0.10)were higher than those in control group(1.38±0.10),P<0.01;and the level of serum HC50 of MPP 0.22g/kgBW group(141.00±23.00)and 0.45 g/kgBW group(130.40±33.20) were greater than the control(100.30±19.40),P<0.01.The activity of NK cells in MPP 0.22 g/kgBW group(1.672±0.142)was significantly elevated in comparison with this value in control group(1.392±0.182),P<0.05.The percentoge of CD4+T helper(Th)cell in spleen of MPP 0.22.g/kgBW group (32.84±3.776)%and 0.45g/kgBW group(32.42±3.507)% was higher than those in control group (25.06±0.354)%,P<0.05.The concentrations of IL-2 in serum of MPP 0.22g/kgBW group 181.06 pg/ml,0.45 g/kgBW group 94.84 pg/ml and 1.35 g/kgBW group 102.61 pg/ml were higher than those incontrol group 0.50 pg/ml,P<0.05;and the level of IL-5 of MPP 0.22g/kgBW group(38.31)pg/ml was greater than the control 0.50 pg/ml,P<0.05.Nevertheless,no obvious effects on weight increasing.the raho of immune organ and body weight and phagocytosis capacity were observed in our study.Conclusion MPP could improve the immune functions in mice,and might be by the mechanism of enhancing the function of Th cells stimulating the secretion of Thl and Th2 type cell cytokines.