CAJ | 학술논문

对生长在添加有不同浓度的葡萄糖、硫代硫酸钠培养基中的蓝细菌Synechocystis sp.PCC 6803中的甘油酯及其脂肪酸组成进行比较.结果表明:硫代硫酸钠能有效地增加膜脂中硫代异鼠李糖二酰基甘油(SQDG)和磷脂酰甘油(PG)的百分含量,培养基中同时添加葡萄糖时能抵消硫代硫酸钠的这一效应.此外,硫代硫酸钠能显著增加单半乳糖甘油二酯(MGDG)、双半乳糖甘油二酯(DGDG)中十六碳酸(C16:0)的百分含量,这一效应也能为葡萄糖消除.硫代硫酸钠不能显著地改变SQDG中C16:0的百分含量,加入葡萄糖时能降低C16:0的百分含量.这些结果说明硫代硫酸钠可能充当一种还原剂使膜脂处于一种低的不饱和状态,同时加入葡萄糖时能降低硫代硫酸钠的还原力.此外,硫代硫酸钠还可作为SQDG合成中的硫供体.
대생장재첨가유불동농도적포도당、류대류산납배양기중적람세균Synechocystis sp.PCC 6803중적감유지급기지방산조성진행비교.결과표명:류대류산납능유효지증가막지중류대이서리당이선기감유(SQDG)화린지선감유(PG)적백분함량,배양기중동시첨가포도당시능저소류대류산납적저일효응.차외,류대류산납능현저증가단반유당감유이지(MGDG)、쌍반유당감유이지(DGDG)중십륙탄산(C16:0)적백분함량,저일효응야능위포도당소제.류대류산납불능현저지개변SQDG중C16:0적백분함량,가입포도당시능강저C16:0적백분함량.저사결과설명류대류산납가능충당일충환원제사막지처우일충저적불포화상태,동시가입포도당시능강저류대류산납적환원력.차외,류대류산납환가작위SQDG합성중적류공체.
Compositions of glycerolipids and fatty acid compositions of glycerolipids were compared among Synechocystis sp. PCC 6803 cells grown in the BG-11 medium containing different concentrations of glucose and Na2S2O3 in this study. It was found that Na2S2O3 can effectively increase the percentage of sulphoquinovosyl diacylglycerol (SQDG) and phosphatidylglycerol (PG) to total membrane lipids and the simultaneous application of glucose with Na2S2O3 can counteract the effect of Na2S2O3. In addition,Na2S2O3 can significantly increase the percentage of palmitic acid (C, 16:0) in fatty acid composition of monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) and decrease the fatty acid unsaturation degree accordingly, and these effects can also be eliminated by glucose. These results indicate that Na2S2O3 can take as a reductant to make membrane lipids in a low unsaturated state, and the simultaneous application of glucose can decrease the reducing power of Na2S2O3. In addition, Na2S2O3 can take as a sulfur donor for the synthesis of SQDG.