CAJ | 학술논문

针对在检测过程中发现的大米中存在PCR抑制现象,对PCR抑制因子作用机理进行研究.通过把糙米加工成精米和米麸后的实验证明,PCR抑制因子主要存在于糙米的米麸中.研究间接证明PCR抑制因子主要通过抑制Taq聚合酶的酶活来抑制PCR反应.尝试采用多种方法来对大米中的PCR抑制因子进行鉴定,通过非变性电泳(PAGE)和电泳迁移率实验(EMSA)实验排除了大米中的PCR抑制因子是大分子物质.
침대재검측과정중발현적대미중존재PCR억제현상,대PCR억제인자작용궤리진행연구.통과파조미가공성정미화미부후적실험증명,PCR억제인자주요존재우조미적미부중.연구간접증명PCR억제인자주요통과억제Taq취합매적매활래억제PCR반응.상시채용다충방법래대대미중적PCR억제인자진행감정,통과비변성전영(PAGE)화전영천이솔실험(EMSA)실험배제료대미중적PCR억제인자시대분자물질.
PCR inhibitory substances in the genomic DNA extracted from brown rice, polished rice and rice bran were analyzed and the inhibition mechanisms were explored. PCR inhibitors were mainly observed in rice bran. Indirect evidence also proved that PCR reaction was inhibited via the inhibition of Taq polymerase. PAGE and EMSA analysis revealed that macromolecules may not be the PCR inhibitors in rice.