CAJ | 학술논문

为探究CTAB方法提取植物DNA中影响DNA产量和质量的诸多因素,以豫花15号花生嫩叶为材料,采用酚氯仿、1 0000 r/min、氯仿、不剪口、氯仿一步、酚氯仿一步、不摇30 min 7种不同的处理方法纯化其基因组DNA,并通过外观、紫外分光光度法和琼脂糖凝胶电泳对所提取的DNA样品进行检测,比较不同因素对DNA提取质量的影响.在充分考虑得率、蛋白质含量及DNA分子完整性等指数后,总结出酚氯仿纯化豫花15号花生叶片DNA的最适方法.
위탐구CTAB방법제취식물DNA중영향DNA산량화질량적제다인소,이예화15호화생눈협위재료,채용분록방、1 0000 r/min、록방、불전구、록방일보、분록방일보、불요30 min 7충불동적처리방법순화기기인조DNA,병통과외관、자외분광광도법화경지당응효전영대소제취적DNA양품진행검측,비교불동인소대DNA제취질량적영향.재충분고필득솔、단백질함량급DNA분자완정성등지수후,총결출분록방순화예화15호화생협편DNA적최괄방법.
To explore the factors on CTAB method of plant DNA extraction that affect the quantity and quality, seven methods phenol-chloroform, 10 000 r/min, chloroform, no cutting on peptips, one step with chloroform, one step with phenol,no shaking for 30 min have been used to extract genomie DNA from peanut leaves of Arachis hypogaea. The DNA samples obtained by above methods were examed by appearance,agarose gel electrophoresis and ultraviolet spectrophotometry. Based on the comparative analysis of yield and quality of the DNA samples by different methods, consideration all index of yield, protein content and the integrity of DNA molecules,the phenol-chloroform method was found to be the most suitable for extraction of genomic DNA from leaves of A rachis hypogaea.