CAJ | 학술논문

为挖掘用于生物防治的微生物资源,自海南蚕豆枯萎病区土壤分离1株芽孢杆菌HSY-8-1,经抗菌试验分析表明,其代谢产生的拮抗物质对引发植物立枯病和根腐病的立枯丝核菌(Rhizoctonia solani)和镰刀菌(Fusarium spp.)抑菌作用较强,具有使病菌菌丝扭曲、缢缩等致畸效应,并抑制立枯丝核菌的菌核形成.研究表明,在NB液体培养基中发酵时,进入稳定生长期后开始产生抑菌物质,72 h时累积达到最大值.这种抑菌物质的特性为外泌型、水溶性,可透过0.22 μm的微孔滤膜,对热、酸、碱、蛋白酶水解、紫外线照射等影响有较强的稳定性.
위알굴용우생물방치적미생물자원,자해남잠두고위병구토양분리1주아포간균HSY-8-1,경항균시험분석표명,기대사산생적길항물질대인발식물립고병화근부병적립고사핵균(Rhizoctonia solani)화렴도균(Fusarium spp.)억균작용교강,구유사병균균사뉴곡、액축등치기효응,병억제립고사핵균적균핵형성.연구표명,재NB액체배양기중발효시,진입은정생장기후개시산생억균물질,72 h시루적체도최대치.저충억균물질적특성위외비형、수용성,가투과0.22 μm적미공려막,대열、산、감、단백매수해、자외선조사등영향유교강적은정성.
In order to excavate microbial resources for bio-control use, Bacillus sp. HSY-8-1 was isolated from broadbean field samples collected in Hainan. HSY-8-1 and its anti-fungal substance can effectively inhibit Rhizoctonia solani and Fusarium spp. growth. They cause hyphae of Rhizoctonia solani and Fusarium spp. malformation and delay sclerotia of Rhizoctonia solani germination. The study demonstrated that when fermented in NB liquid medium and after the growth entering into the stable phase, it began to produce antifungal substance and accumulated to the maximum at 72 hours. It is a kind of extracellularly secretive, liposoluble, 0.22 μm microporous membrane permeable substance and is stable in various treatments of heat, acid or alkali, enzyme hydrolyzed and UV ray.