CAJ | 학술논문

目的探讨雌激素α、β受体(ERα、ERβ)在雌性大鼠眼葡萄膜组织的表达.方法选择青春期SD雌性大鼠22只,采用颈椎脱臼处死大鼠, 取眼球作常规石蜡包埋,连续切片, SP免疫组织化学方法显示ERα、ERβ在葡萄膜组织的表达;采用Tanaka记分法对ERα、ERβ进行定量分析,并设正常大鼠子宫作阳性对照;PBS代替一抗作阴性对照.同时采用放射免疫分析方法检测大鼠血清雌二醇的浓度.结果 ERβ在虹膜基质细胞、虹膜前后两层色素上皮细胞、睫状体非色素上皮和色素上皮、脉络膜各层血管内皮的表达水平主要呈中表达或高表达;ERα则表达不明显.ERβ阳性表达率明显高于ERα,经统计学分析两者间有显著性差异(P＜0.05).ERα、ERβ免疫阳性反应物呈颗粒状,定位在细胞质或细胞核.正常大鼠血清雌二醇水平经检测含量为(22.13±3.54)ng/L.结论大鼠葡萄膜组织以ERβ表达为主,雌激素主要通过ERβ信号转导途径对这些组织的功能起调控作用.
목적 탐토자격소α、β수체(ERα、ERβ)재자성대서안포도막조직적표체.방법 선택청춘기SD자성대서22지,채용경추탈구처사대서, 취안구작상규석사포매,련속절편, SP면역조직화학방법현시ERα、ERβ재포도막조직적표체;채용Tanaka기분법대ERα、ERβ진행정량분석,병설정상대서자궁작양성대조;PBS대체일항작음성대조.동시채용방사면역분석방법검측대서혈청자이순적농도.결과 ERβ재홍막기질세포、홍막전후량층색소상피세포、첩상체비색소상피화색소상피、맥락막각층혈관내피적표체수평주요정중표체혹고표체;ERα칙표체불명현.ERβ양성표체솔명현고우ERα,경통계학분석량자간유현저성차이(P＜0.05).ERα、ERβ면역양성반응물정과립상,정위재세포질혹세포핵.정상대서혈청자이순수평경검측함량위(22.13±3.54)ng/L.결론 대서포도막조직이ERβ표체위주,자격소주요통과ERβ신호전도도경대저사조직적공능기조공작용.
Objective To research the expression of the estrogen receptor alpha ( Erα) and estrogen receptor beta ( Erβ) in uvea tissues of the female rats, and to provide molecular biology data for further studies of the relation estrogen to uvea diseases. Methods Twenty-two adolescent SD female rats were selected. All rats were killed by dislocation of cervical vertebra, the eyeballs were in paraffin imbedding and made to a series of sections, using immunohistochemical method;Erα and Erβ distribution were investigated in uvea tissue of rats;and quantitied by Tanaka scores analytical method. The uteri of rats was used as positive control and PBS as negative control. The level of estradiol in serum of the rats were examined by radioimmunoassay. Results The expression level of Erβ was moderate or highter in stroma cell, anterior pigment epithelium as well as pasterior pigment epithelium of the iris, unpigmented epithelium, pigmented ciliary epithelium and vascular endocemet of the choroid layers. But Erα was not obviously expressed in uvea tissues. The expression rate of Erβ was higher than Erα in these tissues(P＜0.05). Immnoreactivity positive substance was granule, which was distributed in the cytoplasm or in the nucleus. The level of estradiol in serum of the rats was (22.13±3.54)ng/L.Conclusion The expression of either Erα or Erβ in uvea tissues of rats is mainly in Erβ. The results indicate that uvea tissue is regulated directly by estrogen throught Erβ.